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AQUAPORIN 4 EXPRESSION AND DISTRIBUTION DURING OSMOTIC BRAIN EDEMA AND FOLLOWING CHRONIC TREATMENT OF DESIPRAMINE

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2011, Master of Science (MS), Wright State University, Anatomy.
Osmotic brain edema or chronic treatment with desipramine alters brain water permeability. In this study we investigated aquaporin 4 expression and distribution in these two conditions. Brain edema development was induced by intraperitoneal water injection. Blood serum osmolality decreased from 296 ± 1 mOsm to 278 ± 2 mOsm within 15 min. Cerebral cortex water content increased from 79.8 ± 0.2 % to 81.3 ± 0.5% during 120 min of this hyposmotic exposure. Aquaporin 4 immunostaining intensity at the astrocytic endfeet increased in water injected animals from 2.6 ± 0.04 intensity unites (IU) to 3.2 ± 0.21 IU, while total brain AQP4 expression remained unaltered. Chronic treatment with desipramine showed no significant change in serum osmolality or cerebral cortex water content. Similar to water injected animals, aquaporin 4 immunostaining intensity in chronic desipramine animals increased at the astrocytic endfeet (2.5 ± 0.04 IU to 3.0 ± 0.13) but total cortical AQP4 expression was again unaltered. These experiments suggest that decreased brain water permeability caused by osmotic brain edema is not a result of decreased AQP4 expression or localization of astroglial endfeet. However chronic treatment with desipramine increases AQP4 immunostaining at the astrocytic endfeet without increasing overall AQP4 expression.
James Olson, PhD (Committee Chair)
Christopher Wyatt, PhD (Committee Member)
John Pearson, PhD (Committee Member)
120 p.

Recommended Citations

Citations

  • Robinson, S. A. (2011). AQUAPORIN 4 EXPRESSION AND DISTRIBUTION DURING OSMOTIC BRAIN EDEMA AND FOLLOWING CHRONIC TREATMENT OF DESIPRAMINE [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1313150441

    APA Style (7th edition)

  • Robinson, Sergei. AQUAPORIN 4 EXPRESSION AND DISTRIBUTION DURING OSMOTIC BRAIN EDEMA AND FOLLOWING CHRONIC TREATMENT OF DESIPRAMINE. 2011. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1313150441.

    MLA Style (8th edition)

  • Robinson, Sergei. "AQUAPORIN 4 EXPRESSION AND DISTRIBUTION DURING OSMOTIC BRAIN EDEMA AND FOLLOWING CHRONIC TREATMENT OF DESIPRAMINE." Master's thesis, Wright State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=wright1313150441

    Chicago Manual of Style (17th edition)