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Regulation of the stability and cellular localization of the Coxsackievirus and adenovirus receptor (CAR)

Yan, Ran
http://rave.ohiolink.edu/etdc/view?acc_num=wright1369675943

Abstract Details

2013, Master of Science (MS), Wright State University, Biological Sciences.
In polarized epithelia, the seven exon isoform of the Coxsackievirus and adenovirus receptor (CAREx7) is a basolateral cell-cell adhesion protein that is inaccessible for viral infection. In contrast, the eight-exon CAR isoform (CAREx8) localizes at the apical surface and mediates adenovirus (AdV) infection. A PDZ-domain containing protein, MAGI-1, interacts with both isoforms of CAR. I hypothesized that each CAR isoform interacts with specific MAGI-1 PDZ domain(s). Co-immunoprecipitation, FRET and binding assays showed that CAREx7 and CAREx8 both interact with MAGI-1-PDZ3 with high affinity. CAREx8 also interacts with MAGI-1-PDZ1. Whereas the CAREx7-PDZ3 interaction regulates MAGI-1 junction localization, PDZ3 suppresses CAREx8 cell surface levels and AdV infection. Surprisingly, PDZ1 can rescue CAREx8 from MAGI-1-mediated degradation. I also hypothesized that MAGI-1 directs CAREx8 to the ER-associated degradation (ERAD) pathway. Inhibitor experiments demonstrated that ERAD-UPS is associated with MAGI-1-mediated CAREx8 degradation. These novel findings provide insight into the stability and cellular regulation of CAR.
Katherine Excoffon, Ph.D. (Advisor)
Heather Hostetler, Ph.D. (Committee Member)
Scott Baird, Ph.D. (Committee Member)
111 p.
Biology

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Citations

  • Yan, R. (2013). Regulation of the stability and cellular localization of the Coxsackievirus and adenovirus receptor (CAR) [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1369675943

    APA Style (7th edition)

  • Yan, Ran. Regulation of the stability and cellular localization of the Coxsackievirus and adenovirus receptor (CAR). 2013. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1369675943.

    MLA Style (8th edition)

  • Yan, Ran. "Regulation of the stability and cellular localization of the Coxsackievirus and adenovirus receptor (CAR)." Master's thesis, Wright State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=wright1369675943

    Chicago Manual of Style (17th edition)

wright1369675943
275
© 2013, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.