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Triet M Truong MS thesis final.pdf (1.09 MB)

ETD Abstract Container

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Investigating DNA barcoding potentials and genetic structure in Ozobranchus spp. from Atlantic and Pacific ocean sea turtles

Truong, Triet Minh
http://rave.ohiolink.edu/etdc/view?acc_num=wright1392769367

Abstract Details

2014, Master of Science (MS), Wright State University, Chemistry.
The Ozobranchidae family is the smallest and least studied hirudinean taxon. Our research includes the largest molecular dataset yet reported for marine ozobranchids (Ozobranchus margoi and Ozobranchus branchiatus) with the most number of documented turtle hosts (57) from the Atlantic and Pacific Oceans to date of any marine turtle epibiont study. Turtle species sampled in this study include loggerheads (Caretta caretta), hawksbill (Eretmochelys imbricata), olive ridley (Lepidochelys olivacea), and green turtles (Chelonia mydas). Phylogenetic analyses of mitochondrial (COI) and nuclear ribosomal (18S and 28S) genes all support the monophyly of marine Ozobranchidae leeches with speciation occurring over an extensive period of time, likely prior to the Isthmus of Panama. Histone H3 data suggests at least three histone H3 genes for O. margoi. In addition, mtDNA analyses show higher genetic structure in the Atlantic for O. branchiatus existing in both ocean basins. The small tropical family of turtle annelids was also used to examine the limitations of DNA barcoding on taxa with incomplete taxonomic sampling and to assess whether these issues can be adequately resolved using the character-based approach. The ability to assign ocean basin origin of leech specimens using character-based DNA barcoding suggests the potential for this tool to be integrated with other applications besides species identification.
Audrey McGowin, Ph.D. (Advisor)
Daniel Ketcha, Ph.D. (Committee Member)
Ioana Sizemore, Ph.D. (Committee Member)
79 p.
Conservation; Ecology; Genetics; Molecular Biology; Parasitology
Ozobranchus branchiatus, Ozobranchus margoi, marine turtles, DNA barcoding, genetic structure

Recommended Citations

Citations

  • Truong, T. M. (2014). Investigating DNA barcoding potentials and genetic structure in Ozobranchus spp. from Atlantic and Pacific ocean sea turtles [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1392769367

    APA Style (7th edition)

  • Truong, Triet. Investigating DNA barcoding potentials and genetic structure in Ozobranchus spp. from Atlantic and Pacific ocean sea turtles . 2014. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1392769367.

    MLA Style (8th edition)

  • Truong, Triet. "Investigating DNA barcoding potentials and genetic structure in Ozobranchus spp. from Atlantic and Pacific ocean sea turtles ." Master's thesis, Wright State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=wright1392769367

    Chicago Manual of Style (17th edition)

wright1392769367
1,688
© 2014, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.