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The Modulatory Role of Circulating Microvesicles in Endothelial Progenitor Cell Function Is Altered in T2DM.

Ammar, Hala Mustafa
http://rave.ohiolink.edu/etdc/view?acc_num=wright1401458766

Abstract Details

2014, Master of Science (MS), Wright State University, Pharmacology and Toxicology.
Circulating microvesicles (cMVs) are the extracellular MVs released from the cells in the blood and on the vascular wall. Our previous study demonstrates that cMVs of diabetic mouse are detrimental to endothelial progenitor cells (EPCs), which are known to be very important for maintaining normal endothelial function and structure. In this study, we compared the levels of circulating EPCs and EPC-derived MVs (EPC-MVs) in diabetic and healthy subjects. Also, the migration ability, apoptosis rate and reactive oxygen species (ROS) production of EPCs cultured from diabetic and healthy subjects were determined. More importantly, we evaluated whether cMVs from healthy subjects (ch-MVs) improves the function of EPCs from diabetic patients (d-EPCs), and whether cMVs from diabetic patients (cd-MVs) impairs the function of EPCs from healthy subjects (h-EPCs). The d-EPCs or h-EPCs were incubated with ch-MVs or cd-MVs for 24 hours. The migration ability of EPCs was analyzed by an assay kit. The apoptotic rate and ROS production were analyzed by labeling with propidium iodide (PI) and dihydroethidium (DHE) respectively, followed with flow cytometeric analysis. Our data showed that (1) there was a decrease in EPCs iv and an elevation in EPC-MVs in diabetic patients when compared to healthy subjects; (2) The migration ability of d-EPCs were decreased, and the apoptosis rate and ROS production were increased in d-EPCs; (3) ch-MVs improve the function of d-EPC through improving its migration ability and decreasing the apoptosis and ROS production; (4) cd-MVs increase h-EPC apoptosis, and increase ROS production. We conclude that cMVs modulate EPC function and this role of cMVs is reversed in diabetes with the mechanism linked to ROS production.
Yanfang Chen, M.D., Ph.D. (Advisor)
David Cool, Ph.D. (Committee Member)
Ji C. Bihl, M.D., Ph.D. (Committee Member)
66 p.
Medicine; Pharmacology; Toxicology
Extracellular microvesicles, endothelial progenitor cells,EPCs , diabetes, flow cytometer,

Recommended Citations

Citations

  • Ammar, H. M. (2014). The Modulatory Role of Circulating Microvesicles in Endothelial Progenitor Cell Function Is Altered in T2DM. [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1401458766

    APA Style (7th edition)

  • Ammar, Hala. The Modulatory Role of Circulating Microvesicles in Endothelial Progenitor Cell Function Is Altered in T2DM. 2014. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1401458766.

    MLA Style (8th edition)

  • Ammar, Hala. "The Modulatory Role of Circulating Microvesicles in Endothelial Progenitor Cell Function Is Altered in T2DM." Master's thesis, Wright State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=wright1401458766

    Chicago Manual of Style (17th edition)

wright1401458766
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This open access ETD is published by Wright State University and OhioLINK.