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WSU_MS_THESIS_TRISHA BROCKMAN_2014.pdf (20.54 MB)

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Binding and entry mechanisms of adenovirus in polarized epithelial cells

Brockman, Trisha Lynn
http://rave.ohiolink.edu/etdc/view?acc_num=wright1410214584

Abstract Details

2014, Master of Science (MS), Wright State University, Biological Sciences.
Adenovirus’s primary receptor, the Coxsackievirus and adenovirus receptor (CAR), has two transmembrane isoforms with differential localization within polarized epithelial cells (CAREx7 (basolateral), CAREx8 (apical)). I hypothesized that each isoform of CAR is degraded at different rates and is regulated by Src-family kinases. Increasing or decreasing the concentration of CAR is predicted to directly alter adenoviral entry. To test this hypothesis, inducible Madin Darby Canine Kidney (MDCK) cell lines either expressing CAREx7, CAREx8, or mCherry, under a doxycycline-inducible promoter were used in pulse-chase structured experiments to calculate the half-lives of these proteins. Alternatively, a polarized model lung epithelium (CaLu-3) treated with Src-kinase inhibitor, PP2, was used to test apical CAREx8 expression and viral transduction. Though CAREx7 and CAREx8 did have varying half-lives (5.1 h, 2.0 h respectively), inhibiting Src-family kinases did not decrease CAREx8 expression at early time points but did diminish adenovirus transduction. These data confirm that Src does not play a role in CAR turnover, but remains critical for adenoviral infection.
Katherine Excoffon, Ph.D. (Advisor)
Lynn Hartzler, Ph.D. (Committee Member)
Mill Miller, Ph.D. (Committee Member)
112 p.
Biochemistry; Biology; Cellular Biology
Adenovirus; CAR; Coxsackievirus and adenovirus receptor; Src-family kinases; Half-life

Recommended Citations

Citations

  • Brockman, T. L. (2014). Binding and entry mechanisms of adenovirus in polarized epithelial cells [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1410214584

    APA Style (7th edition)

  • Brockman, Trisha. Binding and entry mechanisms of adenovirus in polarized epithelial cells. 2014. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1410214584.

    MLA Style (8th edition)

  • Brockman, Trisha. "Binding and entry mechanisms of adenovirus in polarized epithelial cells." Master's thesis, Wright State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=wright1410214584

    Chicago Manual of Style (17th edition)

wright1410214584
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© 2014, some rights reserved.Creative Commons License Binding and entry mechanisms of adenovirus in polarized epithelial cells by Trisha Lynn Brockman is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Wright State University and OhioLINK.