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COMPARISON BETWEEN FLOW CYTOMETRY AND BEAD METHOD IN COUNTING CD4 AND CD8 T LYMPHOCYTES IN MOUSE SPLEEN CELLS SUSPENSION

Allabidi, Abdulrahman Abdalla

Abstract Details

2014, Master of Science (MS), Wright State University, Microbiology and Immunology.
The flow cytometry is gold standard method to count CD4, CD8 human T lymphocytes, and calculate CD8 to CD4 ratio in HIV patients peripheral blood to stage them and monitor their response to treatment. In this study, a comparison was made by counting CD4and CD8 mouse spleen T lymphocytes by flow cytometry, and a method involving antibody coated latex beads. The bead method yielded results comparable to those obtained by flow cytometry. These results indicate that antibody coated beads are suitable to determine CD4 and CD8 + T lymphocytes counts in situation where flow cytometry is not readily available.
Nancy Bigley, Ph.D. (Advisor)
Barbara Hull, Ph.D. (Committee Member)
Cheryl Conley, Ph.D. (Committee Member)
73 p.

Recommended Citations

Citations

  • Allabidi, A. A. (2014). COMPARISON BETWEEN FLOW CYTOMETRY AND BEAD METHOD IN COUNTING CD4 AND CD8 T LYMPHOCYTES IN MOUSE SPLEEN CELLS SUSPENSION [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1421242306

    APA Style (7th edition)

  • Allabidi, Abdulrahman. COMPARISON BETWEEN FLOW CYTOMETRY AND BEAD METHOD IN COUNTING CD4 AND CD8 T LYMPHOCYTES IN MOUSE SPLEEN CELLS SUSPENSION. 2014. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1421242306.

    MLA Style (8th edition)

  • Allabidi, Abdulrahman. "COMPARISON BETWEEN FLOW CYTOMETRY AND BEAD METHOD IN COUNTING CD4 AND CD8 T LYMPHOCYTES IN MOUSE SPLEEN CELLS SUSPENSION." Master's thesis, Wright State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=wright1421242306

    Chicago Manual of Style (17th edition)