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Application of Fluorescence in situ Hybridization for Visualization and Quantification of Human Gastrointestinal Microbiota

Gordon, Alexander M.
http://rave.ohiolink.edu/etdc/view?acc_num=wright1440846338

Abstract Details

2015, Master of Science (MS), Wright State University, Microbiology and Immunology.
The microbiota of the human gastrointestinal tract is the focus of current research due to their role in human health and disease. Modern methods characterize the communities of gut microbiota through the use of culture independent techniques. Technologies such as microarrays and next generation sequencing (NGS) determine microbial profiles by analyzing the pool of 16S ribosomal small subunit RNA genes in the community. These techniques operate through the measurement of genomic content rather than through direct analysis of cells. This approach predisposes the methods to discrepancies and bias. In contrast, the use of fluorescence in situ hybridization (FISH) to analyze the gut microbiota allows for the visualization of the cells as well as determination of relative abundances of target taxa. This work describes the application of FISH to visualize the relative abundances of several different members of human gut microbiota between select samples. The results showed that the abundance data obtained from FISH are similar to data collected with microarray and NGS techniques. FISH data demonstrated that there is an enrichment of class Bacilli and genus Prevotella in the gastrointestinal tract of Egyptian children, while children from the U.S. show a higher level of genus Bacteroides. This study also showed that the abundances of class Bacteroidia and genus Bifidobacterium fluctuate in adults undergoing a diet intervention program replacing typical diets with high-protein or high-fat diets. In conclusion, this work illustrates the capabilities of FISH to visualize the members of human gut microbiota and quantify relative abundances of target taxa that are a part of a larger community.
Oleg Paliy, Ph.D. (Advisor)
Barbara Hull, Ph.D. (Committee Member)
Shulin Ju, Ph.D. (Committee Member)
46 p.
Biomedical Research; Microbiology
Gastrointestinal Microbiota; Fluorescence in situ Hybridization; FISH

Recommended Citations

Citations

  • Gordon, A. M. (2015). Application of Fluorescence in situ Hybridization for Visualization and Quantification of Human Gastrointestinal Microbiota [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1440846338

    APA Style (7th edition)

  • Gordon, Alexander. Application of Fluorescence in situ Hybridization for Visualization and Quantification of Human Gastrointestinal Microbiota. 2015. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1440846338.

    MLA Style (8th edition)

  • Gordon, Alexander. "Application of Fluorescence in situ Hybridization for Visualization and Quantification of Human Gastrointestinal Microbiota." Master's thesis, Wright State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=wright1440846338

    Chicago Manual of Style (17th edition)

wright1440846338
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© 2015, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.