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Amal Alajman.pdf (1.74 MB)

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Comparing Antibody-Coated Immune Beads With Flow Cytometry to Measure ß-2-Microglubulin+ Murine Spleen Cells.

Alajman, Amal Fahad
http://rave.ohiolink.edu/etdc/view?acc_num=wright1464188382

Abstract Details

2016, Master of Science (MS), Wright State University, Microbiology and Immunology.
In diseases like AIDS, Multiple Myeloma, Multiple Sclerosis (MS), Type 1 Diabetes, and Systemic Lupus Erythromatosus the detection of major histocompatibility complex type 1 molecules (MHC 1) can be a helpful component of disease diagnosis and prognosis. Most somatic mammalian cells display to varying degrees major histocompatibility complex (MHC) class 1 antigen on the cell surface. MHC1 molecules consist of a polymorphic alpha a chain and a monomorphic beta chain, beta 2 microglubulin (ß2m). The ß2M composition is fairly constant within a species whereas the alpha chain is not. The polymorphic alpha chain is encoded by an MHC complex gene that is specific for each member of the species. The consistency of the ß2M protein within a species makes it a superior target to the variable a chains when attempting to quantify MHC1 molecules. Currently, there are no reagents available to identify the MHC1 antigen within an outbred population such as human or laboratory animals other than defined in bred mouse strains. In this study, the presence of MHC1 molecules on spleen cells from an outbred mouse strain (ICR strain) was evaluated by looking for monomorphic ß2m molecules. The two methods, which were used to obtain the numbers of ß2m+ mouse spleen cells, were flow cytometry and the antibody-latex bead method. Similar numbers of ß2m- positive cells were obtained using these two methods (7-9%). These results demonstrate that the antibody-coated beads are a suitable, less expensive and time saving alternative to determine the numbers of ß2m+ positive cells when compared to flow cytometry.
Nancy Bigley, Ph.D. (Advisor)
Barbara Hull, Ph.D. (Committee Member)
Dawn Wooley, Ph.D. (Committee Member)
60 p.
Immunology; Microbiology
microbiology; immunology

Recommended Citations

Citations

  • Alajman, A. F. (2016). Comparing Antibody-Coated Immune Beads With Flow Cytometry to Measure ß-2-Microglubulin+ Murine Spleen Cells. [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1464188382

    APA Style (7th edition)

  • Alajman, Amal. Comparing Antibody-Coated Immune Beads With Flow Cytometry to Measure ß-2-Microglubulin+ Murine Spleen Cells. 2016. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1464188382.

    MLA Style (8th edition)

  • Alajman, Amal. "Comparing Antibody-Coated Immune Beads With Flow Cytometry to Measure ß-2-Microglubulin+ Murine Spleen Cells." Master's thesis, Wright State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=wright1464188382

    Chicago Manual of Style (17th edition)

wright1464188382
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© 2016, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.