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Determining Protein-Protein Interactions of ALS-Associated SOD1

Shurte, Leah A.
http://rave.ohiolink.edu/etdc/view?acc_num=wright1464283630

Abstract Details

2016, Master of Science (MS), Wright State University, Biological Sciences.
Amyotrophic Lateral Sclerosis (ALS) is a neurodegenerative disorder that occurs due to the death of motor neurons and leads to paralysis and death within three to five years after symptoms present (Byrne et al., 2013). Superoxide Dismutase 1 (SOD1) was first identified to be associated with ALS in 1993. The objective of this study is to determine which proteins interact with wild type and mutant SOD1 and find any similarities or differences between them. ALS is attributed to a gain of toxicity, therefore abnormal protein interactions in mutant SOD1 are important. The results of this study will provide insight on the protein-protein interactions of SOD1, as well as how important these interactions are in association with ALS. Initially, the plan was to use yeast two-hybrid screening (Y2H) to identify the protein-protein interactions, then confirm the interactions with a pull down assay (immunoprecipitation). However, the Y2H was unable to obtain results. Instead, a combination of a pull-down assay and mass spectrometry were used to identify protein-protein interactions. Fifty one proteins were identified to interact exclusively with wild type SOD1 and thirteen proteins interacted with both wild type and A4V SOD1.
Shulin Ju, Ph.D. (Advisor)
Mill Miller, Ph.D. (Committee Member)
Paula Bubulya, Ph.D. (Committee Member)
Quan Zhong, Ph.D. (Committee Member)
72 p.
Biology; Cellular Biology
SOD1; Superoxide Dismutase 1; ALS; Amyotrophic Lateral Sclerosis; Protein-Protein Interactions

Recommended Citations

Citations

  • Shurte, L. A. (2016). Determining Protein-Protein Interactions of ALS-Associated SOD1 [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1464283630

    APA Style (7th edition)

  • Shurte, Leah. Determining Protein-Protein Interactions of ALS-Associated SOD1. 2016. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1464283630.

    MLA Style (8th edition)

  • Shurte, Leah. "Determining Protein-Protein Interactions of ALS-Associated SOD1." Master's thesis, Wright State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=wright1464283630

    Chicago Manual of Style (17th edition)

wright1464283630
367
© 2016, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.