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MaryKThompsonJenkinsThesis082616.pdf (850.39 KB)

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Metabolism of Herpes Simplex Virus 1 infected RAW 264.7 macrophages

Jenkins, Mary K.
http://rave.ohiolink.edu/etdc/view?acc_num=wright1472203113

Abstract Details

2016, Master of Science (MS), Wright State University, Microbiology and Immunology.
Macrophages are immune cells that phagocytize pathogens and act as antigen presenting cells. Macrophages are critical in regulating the adaptive immune response and play a key role in neutralizing infection. Macrophages exhibit a variety of phenotypes which have different energy requirements. In the case of HSV-1 infection the virus has been shown to alter metabolic processes of its host. Utilizing a XF24 Extracellular Flux Analyzer the metabolic activity of M0, M1, and M2 (IL-4, IL-13, and IL-10) RAW 264.7 macrophages was quantified for both uninfected cells and in response to HSV-1 infection. The analysis showed uninfected M0 and M2 (IL-4, IL-13, IL-10) macrophages undergo glycolysis and mitochondrial respiration for energy metabolism while M1 macrophages only undergo glycolysis. HSV-1 showed the ability to alter metabolism in macrophages by inhibiting oxidative phosphorylation. This inhibition leads to increased rates of glycolysis with variability shown among the macrophage phenotypes. As HSV-1 is dependent upon its host cell for energy production the degree of response can be correlated to viral replication.
Nancy Bigley, Ph.D. (Committee Chair)
Debra Mayes, Ph.D. (Committee Member)
Courtney Sulentic, Ph.D. (Committee Member)
69 p.
Biology; Immunology; Microbiology
immunology; biology; microbiology

Recommended Citations

Citations

  • Jenkins, M. K. (2016). Metabolism of Herpes Simplex Virus 1 infected RAW 264.7 macrophages [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1472203113

    APA Style (7th edition)

  • Jenkins, Mary. Metabolism of Herpes Simplex Virus 1 infected RAW 264.7 macrophages. 2016. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1472203113.

    MLA Style (8th edition)

  • Jenkins, Mary. "Metabolism of Herpes Simplex Virus 1 infected RAW 264.7 macrophages." Master's thesis, Wright State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=wright1472203113

    Chicago Manual of Style (17th edition)

wright1472203113
584
© 2016, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.