Skip to Main Content
Frequently Asked Questions
Submit an ETD
Global Search Box
Need Help?
Keyword Search
Participating Institutions
Advanced Search
School Logo
Files
File List
TITLE PAGE AND APPROVAL SHEET copy 2.pdf (4.8 MB)
ETD Abstract Container
Abstract Header
Instability at Trinucleotide Repeat DNAs
Author Info
Gadgil, Rujuta Yashodhan
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=wright1472231204
Abstract Details
Year and Degree
2016, Master of Science (MS), Wright State University, Biochemistry and Molecular Biology.
Abstract
Trinucleotide repeats (TNRs) are sequences prone to formation of non-B DNA structures and mutations; undergo expansions in vivo to cause various inherited neurodegenerative diseases. Hairpin structures formed during DNA replication or repair can cause replication fork stalling and if left unrepaired could cause single or double strand DNA breaks. To test and study this hypothesis we have devised a novel two color marker gene assay to detect DNA breaks at TNRs. By inducing replication stress our results show that TNRs are prone to DNA strand breaks and it is dependent on the repeat tract length. Double strand breaks at structured DNA are repaired differently than `clean’ DSBs. The cells which undergo breaks die off, possibly due to inability to repair breaks. Translesion polymerases help tolerate DNA damage at TNR region.
Committee
Michael Leffak, Ph.D. (Advisor)
John Paietta, Ph.D. (Committee Member)
Michael Markey, Ph.D. (Committee Member)
Pages
108 p.
Subject Headings
Biochemistry
;
Molecular Biology
Keywords
Tri-nucleotide
;
repeats
;
non-B DNA
;
structures
;
inherited
;
neurodegenerative
;
replication
;
fork
;
stalling
;
single
;
double
;
strand
;
DNA
;
breaks
;
color
;
marker
;
gene
;
assay
;
detect
;
DNA
;
breaks
Recommended Citations
Refworks
EndNote
RIS
Mendeley
Citations
Gadgil, R. Y. (2016).
Instability at Trinucleotide Repeat DNAs
[Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1472231204
APA Style (7th edition)
Gadgil, Rujuta Yashodhan.
Instability at Trinucleotide Repeat DNAs.
2016. Wright State University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=wright1472231204.
MLA Style (8th edition)
Gadgil, Rujuta Yashodhan. "Instability at Trinucleotide Repeat DNAs." Master's thesis, Wright State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=wright1472231204
Chicago Manual of Style (17th edition)
Abstract Footer
Document number:
wright1472231204
Download Count:
428
Copyright Info
© 2016, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.