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FINAL - DIVYA CHEEDU THESIS pdf.pdf (1.78 MB)

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Regulation of Mitotic Progression by Btf and TRAP150

Cheedu, Divya
http://rave.ohiolink.edu/etdc/view?acc_num=wright1484576274843721

Abstract Details

2016, Master of Science (MS), Wright State University, Biological Sciences.
Serine-arginine-rich (SR) or SR-like splicing factors interact with exon junction complex proteins during pre-mRNA processing to promote mRNA packaging into mature messenger ribonucleoproteins (mRNPs) and to dictate mRNA stability, nuclear export, and translation. In this thesis, I examined if depletion of two homologous non-classical serine-arginine-rich (SR) splicing factors, Btf (BCLAF1) and TRAP150, impacts regulation of cell cycle regulator transcripts and mitosis. Previous work showed that depletion of these proteins by RNAi causes mitotic defects including chromosome misalignment in metaphase. However, since Btf and/or TRAP150 did not co-localize with mitotic structures during mitosis, I hypothesized that Btf and/or TRAP150 depletion affect mitosis indirectly through altered expression of mitotic regulator transcripts. My results indicated that Btf and TRAP150 are important for controlling the abundance of transcripts that encode mitotic regulators. My results also suggest that Btf and TRAP150 have increased effects in maintaining the alignment of chromosomes at metaphase, as co-depletion of Btf and TRAP150 showed increased effects on the misalignment of chromosomes, thus demonstrating an important role in mitotic progression. Future studies will determine if altered expression or processing of endogenous cell cycle regulator protein transcripts is a result of changes in transcript distribution, and they will tease apart the molecular mechanisms underlying defective mitotic chromosome alignment/segregation in absence of Btf and TRAP150.
Paula Bubulya, Ph.D. (Advisor)
Katherine Excoffon, Ph.D. (Committee Member)
Labib Rouhana, Ph.D. (Committee Member)
96 p.
Biology
SR proteins; Btf; TRAP150; Splicing; Mitosis; Cell-cycle regulator transcripts

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Citations

  • Cheedu, D. (2016). Regulation of Mitotic Progression by Btf and TRAP150 [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1484576274843721

    APA Style (7th edition)

  • Cheedu, Divya. Regulation of Mitotic Progression by Btf and TRAP150 . 2016. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1484576274843721.

    MLA Style (8th edition)

  • Cheedu, Divya. "Regulation of Mitotic Progression by Btf and TRAP150 ." Master's thesis, Wright State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=wright1484576274843721

    Chicago Manual of Style (17th edition)

wright1484576274843721
628
© 2016, some rights reserved.Creative Commons License Regulation of Mitotic Progression by Btf and TRAP150 by Divya Cheedu is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Wright State University and OhioLINK.