Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


Sibomana_Dissertation_PhD.pdf (2.96 MB)

ETD Abstract Container

Abstract Header

Evidence That Myo-Inositol Plus Ethanolamine Elevates Plasmalogen Levels And Lends Protection Against Oxidative Stress In Neuro-2A Cells

Sibomana, Isaie
http://rave.ohiolink.edu/etdc/view?acc_num=wright1484661880801698

Abstract Details

2016, Doctor of Philosophy (PhD), Wright State University, Biomedical Sciences PhD.
Plasmalogens are glycerophospholipids abundant in brain and heart tissues. Evidence suggests that they have antioxidant properties. Studies from our laboratory showed that rats treated with myo-inositol plus ethanolamine (ME) have elevated ethanolamine plasmalogens (PE-Pls) in brain and are protected against phosphine-induced oxidative stress. We hypothesized that ME elevates PE-Pls levels and protects against oxidative stress through oxidation of its vinyl ether bond. We tested this hypothesis in Neuro-2A cell culture and assessed the effects of treatments with myo-inositol (M), ethanolamine (Etn), or a combination (ME) on the: (1) effects on phospholipid (PL) classes, especially Etn PLs; (2) effects on cell viability in response to H2O2-induced oxidative stress; and (3) molecular species of Etn PLs preferentially affected by ME and H2O2 treatments, especially PE-Pls and their degradation byproducts – lyso-phosphatidylethanolamines (LPE). 31P NMR data show that treating the cells with equimolar amounts (500 uM) of M or Etn for 24 h did not influence PL levels, but ME yielded a 3-fold increase in both PE-Pls and PE (p<0.001). Cells exposed to 650 uM H2O2 for 24 h decreased cell viability to 53% ± 1.7. While pretreatment with M or ME significantly increased cell survival to 62% ± 1.2 or 80% ± 0.6, respectively (p<0.05), Etn alone had no effect. Mass spectrometry showed that ME preferentially elevated the levels of PE-Pls species containing saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) by 60%, while PE-Pls containing polyunsaturated fatty acids (PUFA) increased by only 10%. H2O2 caused a significant decrease in PE-Pls (27%), producing a 39% increase in LPE and a 4-fold increase in glycerophosphoethanolamine (GPE), but had no impact on PE levels, suggesting that LPE and GPE were primarily byproducts of PE-Pls degradation. Surprisingly, all these effects were blocked by pre-treating cells with ME prior to H2O2 exposure. Taken together, these data suggest that a preferential increase in PE-Pls species containing SFA+MUFA in response to ME may protect cells from H2O2-induced oxidative stress. The mechanism for this effect is unclear, but further investigations to understand these processes may help to develop neuroprotective approaches to alleviate the progression of neurodegenerative diseases/disorders.
Nicholas Reo, Ph.D. (Advisor)
Nicholas DelRaso, Ph.D. (Committee Member)
Michael Raymer, Ph.D. (Committee Member)
James Olson, Ph.D. (Committee Member)
Jeffery Gearhart, Ph.D. (Committee Member)
174 p.
Biochemistry; Neurosciences
Ethanolamine plasmalogens; oxidative stress; myo-inositol; ethanolamine; Neuro-2A cells; hydrogen peroxide; neuroprotection

Recommended Citations

Citations

  • Sibomana, I. (2016). Evidence That Myo-Inositol Plus Ethanolamine Elevates Plasmalogen Levels And Lends Protection Against Oxidative Stress In Neuro-2A Cells [Doctoral dissertation, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1484661880801698

    APA Style (7th edition)

  • Sibomana, Isaie. Evidence That Myo-Inositol Plus Ethanolamine Elevates Plasmalogen Levels And Lends Protection Against Oxidative Stress In Neuro-2A Cells. 2016. Wright State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1484661880801698.

    MLA Style (8th edition)

  • Sibomana, Isaie. "Evidence That Myo-Inositol Plus Ethanolamine Elevates Plasmalogen Levels And Lends Protection Against Oxidative Stress In Neuro-2A Cells." Doctoral dissertation, Wright State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=wright1484661880801698

    Chicago Manual of Style (17th edition)

wright1484661880801698
903
© 2016, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.