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Differentially Functionalized Silver Nanoparticles Alter K Metabolism in Human Red Blood Cells

Alla, Praveen Kumar
http://rave.ohiolink.edu/etdc/view?acc_num=wright1484693096434035

Abstract Details

2016, Master of Science (MS), Wright State University, Pharmacology and Toxicology.
Silver nano particles (AgNPs) are the most frequently used nanomaterials (NMs) in industrial and biomedical applications, and extensive knowledge about their properties is fast accumulating. In addition, functionalization of nanoparticles significantly impacts their properties and potential applications. In spite the urge to produce, investigate and apply them, the toxicity of nanoparticles and their interaction with blood components, such as erythrocytes, has been insufficiently investigated. Here, we report on the effect of AgNPs in cellular function, and particularly on ion transport in human red blood cells (HRBCs). Two methods of synthesis were used for the functionalization of AgNPs, Creighton, and Lee-Meisel. HRBCs were obtained at a blood bank from blood of adult donors, which was either expired or fresh or further stored refrigerated in the laboratory for variable lengths of time, and from fresh or stored refrigerated cord blood. Rubidium, used as a potassium (K) congener to measure K influx, and the cellular K concentration were determined in the presence and absence of several concentrations (0-150 µg/mL) of negatively charged, spherical 10 nm average diameter AgNPs at different time points (0-60 min). Our findings indicate that Creighton AgNPs, independently of the source, and in a time and dose-dependent manner, inhibited Rb influx and depleted the cells of K ions, although inter-individual differences were observed. In comparison to the Creighton-, the Lee-Meisel-prepared AgNPs caused a maximum Rb inhibition of ~ 50 % and K loss of ~ 15 % with larger inter-individual variability than Creighton AgNPs, albeit in a small sample. The loss of K from HRBCs was entirely accounted for by an increase in K concentration in the extra cellular medium. The effect is apparently due to internalization of AgNPs which can be viewed by integration of enhanced dark field optical microscopy and hyperspectral imaging in a CytoViva® equipment. These findings indicate that functionalization of AgNPs significantly affects HRBC K homeostasis.
Norma Adragna, Ph.D. (Advisor)
Peter Lauf, Ph.D. (Committee Member)
Ioana Sizemore, Ph.D. (Committee Member)
68 p.
Pharmacology
pharmacology

Recommended Citations

Citations

  • Alla, P. K. (2016). Differentially Functionalized Silver Nanoparticles Alter K Metabolism in Human Red Blood Cells [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1484693096434035

    APA Style (7th edition)

  • Alla, Praveen Kumar. Differentially Functionalized Silver Nanoparticles Alter K Metabolism in Human Red Blood Cells . 2016. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1484693096434035.

    MLA Style (8th edition)

  • Alla, Praveen Kumar. "Differentially Functionalized Silver Nanoparticles Alter K Metabolism in Human Red Blood Cells ." Master's thesis, Wright State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=wright1484693096434035

    Chicago Manual of Style (17th edition)

wright1484693096434035
192
© 2016, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.