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Thesisfinaldraft2.docx.pdf (1.87 MB)
ETD Abstract Container
Abstract Header
Thermal Burn Injury Induced Microvesicle Particle Release
Author Info
Fahy, Katherine Erin
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=wright149383031006972
Abstract Details
Year and Degree
2017, Master of Science (MS), Wright State University, Pharmacology and Toxicology.
Abstract
Microvesicle particles (MVP) are found to be important for cellular communication because they contain many bioactive proteins, lipids, cytokines, and nucleic acids. We have previously found that ultraviolet B radiation (UVB) and a Platelet-activating factor agonist (CPAF) can stimulate the release of MVP in keratinocytes. We hypothesized that there may also be an increase in MVP released after thermal burn and that could be involved in pathogenesis of the systemic effects found in some patients. In this thesis various keratinocyte cell lines, mice and human ex vivo skin were used as model systems to test our hypotheses. It was determined that thermal burn significantly increases the release of MVP compared to the untreated groups. UVB, CPAF and thermal burn all seemed to involve acid sphingomyelinase (aSMase), but different MAP kinase pathways. There was also a significant decrease in the cytokine concentration inside MVP after thermal burn, suggesting a possible defense mechanism to prevent cytokine storm.
Committee
Jeffrey Travers, M.D., Ph.D. (Advisor)
David Cool, Ph.D. (Committee Member)
Ji Bihl, M.D., Ph.D. (Committee Member)
Pages
151 p.
Subject Headings
Medicine
;
Pharmacology
Keywords
Microvesicle particles
;
keratinocytes
;
thermal burn
;
ultraviolet B radiation
;
Platelet-activating factor
;
acid sphingomyelinase
;
MAP kinase
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Refworks
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Citations
Fahy, K. E. (2017).
Thermal Burn Injury Induced Microvesicle Particle Release
[Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright149383031006972
APA Style (7th edition)
Fahy, Katherine.
Thermal Burn Injury Induced Microvesicle Particle Release .
2017. Wright State University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=wright149383031006972.
MLA Style (8th edition)
Fahy, Katherine. "Thermal Burn Injury Induced Microvesicle Particle Release ." Master's thesis, Wright State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=wright149383031006972
Chicago Manual of Style (17th edition)
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Document number:
wright149383031006972
Download Count:
624
Copyright Info
© 2017, some rights reserved.
Thermal Burn Injury Induced Microvesicle Particle Release by Katherine Erin Fahy is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Wright State University and OhioLINK.