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Analytical-based methodologies to examine In vitro nanokinetics of silver nanoparticles

Paluri, Sesha Lakshmi Arathi
http://rave.ohiolink.edu/etdc/view?acc_num=wright1496146342673032

Abstract Details

2017, Doctor of Philosophy (PhD), Wright State University, Biomedical Sciences PhD.
Advancements in the nanotechnology have taken a huge leap in 21st century resulting in 1814 consumer products containing nanomaterials. About 47% of these products belong to the health and fitness sector and ~24% utilize silver nanoparticles (AgNPs). Despite the promising biomedical applications of AgNPs (e.g. bone cements, contrasting agents, and drug-carriers), lack of standardized methods for examining their nanokinetics (i.e., Absorption, Distribution, Metabolism, and Elimination (ADMEs)) limit their clinical implementation. The current work addresses this knowledge gap by developing analytical-based approaches for studying in vitro ADMEs of AgNPs. To demonstrate the versatility of these methodologies, two in vitro kidney study models (Vero 76 and HEK 293 cells) were tested under pre-determined exposure concentrations (3-300 µg mL-1) and times (4-48 hr). The ADMEs of both AgNPs+ and AgNPs- in Vero 76 cells were summarized here for illustrative purposes: [A]: Inductively coupled plasma optical emission spectroscopy (ICP-OES) facilitated the evaluation of critical kinetic parameters including order of reaction, rate constant and bioavailability (first-order, kabs= 0.05 hr-1, Cmaximum < 20.7±4% and Tmaximum > 48 hr), [D] CytoViva and Raman imaging outlined the uptake and cellular localization patterns (e.g., Raman results of mapped cells exposed to AgNPs+ and AgNPs- were dominated by the signals corresponding to the plasma membrane and cytoplasm, respectively), [M] Cloud point extraction (CPE) followed by tangential flow filtration enhanced the separation of two Ag species from the cellular matrix (= 11±4% of the AgNPs were converted to Ag+), and [E] ICP-OES also facilitated the construction of clearance-time curves to evaluate the elimination kinetics of sub-lethal AgNPs (first-order, keli=0.039 hr-1). Furthermore, a new laboratory module was developed according to the five essential features laid by the National Research Council for inquiry-based teaching and learning in order to introduce undergraduate and graduate students to the fabrication and characterization of green and non-green silver and gold nanoparticles. As demonstrated by the results of the formative assessments, this hands-on laboratory was not only well-received by students from diverse backgrounds, but also stimulated their critical thinking and helped them acquire new laboratory skills.
Ioana Sizemore, Ph.D. (Committee Chair)
Norma Adragna, Ph.D. (Committee Member)
David Dolson, Ph.D. (Committee Member)
Steven Higgins, Ph.D. (Committee Member)
Mill Miller, Ph.D. (Committee Member)
146 p.
Biomedical Research; Education; Nanoscience
biomedical research; nanoscience; education

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Citations

  • Paluri, S. L. A. (2017). Analytical-based methodologies to examine In vitro nanokinetics of silver nanoparticles [Doctoral dissertation, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1496146342673032

    APA Style (7th edition)

  • Paluri, Sesha Lakshmi. Analytical-based methodologies to examine In vitro nanokinetics of silver nanoparticles . 2017. Wright State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1496146342673032.

    MLA Style (8th edition)

  • Paluri, Sesha Lakshmi. "Analytical-based methodologies to examine In vitro nanokinetics of silver nanoparticles ." Doctoral dissertation, Wright State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=wright1496146342673032

    Chicago Manual of Style (17th edition)

wright1496146342673032
567
© 2017, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.