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Cx43 Expression Increases in Response to Increased Temperature Incubation in the Developing Chicken Embryonic Brain

Barrios, Erick A.
http://rave.ohiolink.edu/etdc/view?acc_num=wright1502963524023006

Abstract Details

2017, Master of Science (MS), Wright State University, Physiology and Neuroscience.
We used the chicken embryo model to examine whether altered incubation temperature during chicken embryo development would alter the protein expression of heat shock protein 70 (hsp70), hsp90, and connexin 43 (cx43) in the brain. We incubated eggs in either optimal (37.6°C, Con), increased (39.6°C, +2), or decreased (35.6°C, -2) temperature conditions until they reached embryonic day 16 (E16). The -2 condition significantly increased incidence of embryonic mortality. As shown in the literature, the development of the eggs was accelerated or decelerated due to increased or decreased temperature respectively. To control for this, we incubated eggs at optimal temperature (37.6°C) to the developmental stage of the +2 (E17.5, HH43-44) and -2 (E12, HH38) conditions. Embryonic chicken brain protein was quantified for hsp70, hsp90, and cx43 using Western Blot analysis. We found that the +2 condition significantly increased cx43 and hsp70 protein expression, but had no effect on hsp90 protein expression.
Debra Mayes, Ph.D. (Advisor)
Adrian Corbett, Ph.D. (Committee Member)
Thomas Brown, Ph.D. (Committee Member)
47 p.
Developmental Biology
developmental biology

Recommended Citations

Citations

  • Barrios, E. A. (2017). Cx43 Expression Increases in Response to Increased Temperature Incubation in the Developing Chicken Embryonic Brain [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1502963524023006

    APA Style (7th edition)

  • Barrios, Erick. Cx43 Expression Increases in Response to Increased Temperature Incubation in the Developing Chicken Embryonic Brain . 2017. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1502963524023006.

    MLA Style (8th edition)

  • Barrios, Erick. "Cx43 Expression Increases in Response to Increased Temperature Incubation in the Developing Chicken Embryonic Brain ." Master's thesis, Wright State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=wright1502963524023006

    Chicago Manual of Style (17th edition)

wright1502963524023006
163
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This open access ETD is published by Wright State University and OhioLINK.