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Akbar, Md Ali. Thesis.pdf (1.59 MB)

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Cloud Point Extraction in Conjunction with Tangential Flow Filtration (CPE-TFF) for the Enhanced Separation of Silver Nanoparticles and Silver Ions from Aqueous Colloids and Biological Matrices

Akbar, Md Ali
http://orcid.org/0000-0003-1270-1501
http://rave.ohiolink.edu/etdc/view?acc_num=wright1515428820139494

Abstract Details

2017, Master of Science (MS), Wright State University, Chemistry.
The extensive use of silver nanoparticles (AgNPs) in consumer products and research is accompanied by their inevitable release into the environment. While the environmental fate of AgNPs is still under investigation, most in vitro and in vivo studies agree that the main toxicity of AgNPs is directly related to the release of Ag+ ions from AgNPs. In this study, cloud point extraction (CPE) was combined with tangential flow filtration (TFF) for the enhanced separation of AgNPs and Ag+ from aqueous colloids and biological matrices. The feasibility of the CPE-TFF method was demonstrated on four colloidal (borohydride-, PVP-, citrate-capped and uncapped AgNPs) and three cellular matrices (Escherichia coli, Vero 76 cells, and mitochondria extracts). The addition of a 1-kD TFF step to CPE was found to boost the total Ag recovery to over 93.4±8.4% for the aqueous colloids and 90.2±8.5% for the mammalian and bacterial cells. The preservation of the size, shape, and optical properties of AgNPs during the CPE-TFF manipulation was confirmed by transmission electron microscopy and UV-Vis absorption spectroscopy. CPE-TFF facilitated the investigation of fundamental nanotoxicity questions such as the redox interconversion of the two Ag species within mitochondria or colloids as a function of time and concentration. CPE-TFF is a simple, time- and cost-effective separation method of AgNPs and Ag+ that could find numerous other applications in the environmental health and safety of nanotechnology area.
Ioana Sizemore, Ph.D. (Advisor)
David Dolson, Ph.D. (Committee Member)
Steven Higgins, Ph.D. (Committee Member)
55 p.
Chemistry
cloud point extraction; tangential flow filtration; silver nanoparticles

Recommended Citations

Citations

  • Akbar, M. A. (2017). Cloud Point Extraction in Conjunction with Tangential Flow Filtration (CPE-TFF) for the Enhanced Separation of Silver Nanoparticles and Silver Ions from Aqueous Colloids and Biological Matrices [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1515428820139494

    APA Style (7th edition)

  • Akbar, Md Ali. Cloud Point Extraction in Conjunction with Tangential Flow Filtration (CPE-TFF) for the Enhanced Separation of Silver Nanoparticles and Silver Ions from Aqueous Colloids and Biological Matrices. 2017. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1515428820139494.

    MLA Style (8th edition)

  • Akbar, Md Ali. "Cloud Point Extraction in Conjunction with Tangential Flow Filtration (CPE-TFF) for the Enhanced Separation of Silver Nanoparticles and Silver Ions from Aqueous Colloids and Biological Matrices." Master's thesis, Wright State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=wright1515428820139494

    Chicago Manual of Style (17th edition)

wright1515428820139494
493
© 2017, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.