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Analysis of Stretch Reflex Responses in Mice Lacking Munc18-1 in Proprioceptors

Mohi, Amr
http://rave.ohiolink.edu/etdc/view?acc_num=wright15160256865382

Abstract Details

2017, Master of Science (MS), Wright State University, Anatomy.
The monosynaptic stretch reflex circuit is one of the simplest circuits of the central nervous system. We studied the connection between Ia proprioceptive afferents and motor neurons that comprise the circuit, by stimulating the dorsal root and recording from the ventral root of the fourth lumbar spinal nerve. In our study, we analyzed the status of neurotransmission in the stretch reflex circuit in postnatal PV-cre; munc18-1 lox/lox conditional mutant mice. Munc18-1 is responsible for synaptic vesicular release in neurons. In the PV-cre; Munc18-1 lox/lox mutants we use, Munc18-1 is only knocked out in proprioceptive afferents and A-beta somatosensory afferents, both of which express parvalbumin (PV). Our results showed that on the first postnatal day (P1), responses evoked from the ventral root are significantly reduced in mutants compared to wild type mice. Similar results were also observed at P6. Our results lead us to conclude that PV-cre; Munc18-1 lox/lox mutant mice lack synaptic vesicular release in proprioceptive afferents after birth as evidenced by a dramatically reduced stretch reflex circuit response analyzed with the electrophysiology experiments in our study.
David Ladle, Ph.D. (Advisor)
Mark Rich, Ph.D. (Committee Member)
Patrick Sonner, Ph.D. (Committee Member)
35 p.
Anatomy and Physiology; Neurosciences
Stretch reflex; Ia afferents; spinal cord circuits; Munc18-1; Cre; proprioceptors; muscle spindles

Recommended Citations

Citations

  • Mohi, A. (2017). Analysis of Stretch Reflex Responses in Mice Lacking Munc18-1 in Proprioceptors [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright15160256865382

    APA Style (7th edition)

  • Mohi, Amr. Analysis of Stretch Reflex Responses in Mice Lacking Munc18-1 in Proprioceptors . 2017. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright15160256865382.

    MLA Style (8th edition)

  • Mohi, Amr. "Analysis of Stretch Reflex Responses in Mice Lacking Munc18-1 in Proprioceptors ." Master's thesis, Wright State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=wright15160256865382

    Chicago Manual of Style (17th edition)

wright15160256865382
449
© 2017, some rights reserved.Creative Commons License Analysis of Stretch Reflex Responses in Mice Lacking Munc18-1 in Proprioceptors by Amr Mohi is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Wright State University and OhioLINK.