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Alyaa Aldebs- Thesis.pdf (1.32 MB)

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Coupling of Mechanical and Electromagnetic Fields Stimulation for Bone Tissue Engineering

Aldebs, Alyaa I.
http://rave.ohiolink.edu/etdc/view?acc_num=wright1527185662564699

Abstract Details

2018, Master of Science in Biomedical Engineering (MSBME), Wright State University, Biomedical Engineering.
Alternative bone regeneration strategies that do not rely on harvested tissue or exogenous growth factors and cells are badly needed. However, creating living tissue constructs that are structurally, functionally and mechanically comparable to the natural bone has been a challenge so far. A major hurdle has been recreating the bone tissue microenvironment using the appropriate combination of cells, scaffold and stimulation to direct differentiation. This project presents a bone regeneration formulation that involves the use of human adipose-derived mesenchymal stems cells (hASCs) and a 3D scaffold based on a self-assembled peptide hydrogel doped with superparamagnetic nanoparticles (NPs). Osteogenic differentiation of hASCs is achieved through the direct stimulation by extremely-low frequency pulsed electromagnetic fields (pEMFs) and the indirect mechanical stimulation, through NP vibration induced by the field. This 3D construct was cultured for up to 21 days and its osteogenic capacity was assessed. Cellular morphology, proliferation, viability, as well as alkaline phosphatase activity, calcium deposition were monitored during this time. The results show that the pEMFs have no negative effect on cell viability and induce early differentiation of hASCs to an osteoblastic phenotype when compared to the cell without biophysical stimulation. This effect results from the synergy between the pEMF and NP that acts as remote stimulation of the mechanotransduction pathways which activate biochemical signals between cells to go under differentiation or proliferation. The use of this approach offers a safe and effective treatment option for the treatment of non-union bone fractures. In addition, this formulation can be directly injected into the wound site, making it minimally invasive as well.
Jaime Ramirez-Vick, Ph.D (Advisor)
Ulas Sunar, Ph.D (Committee Member)
Nasim Nosoudi, Ph.D (Committee Member)
100 p.
Biomedical Engineering
bone regeneration; bone tissue engineering

Recommended Citations

Citations

  • Aldebs, A. I. (2018). Coupling of Mechanical and Electromagnetic Fields Stimulation for Bone Tissue Engineering [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1527185662564699

    APA Style (7th edition)

  • Aldebs, Alyaa . Coupling of Mechanical and Electromagnetic Fields Stimulation for Bone Tissue Engineering. 2018. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1527185662564699.

    MLA Style (8th edition)

  • Aldebs, Alyaa . "Coupling of Mechanical and Electromagnetic Fields Stimulation for Bone Tissue Engineering." Master's thesis, Wright State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=wright1527185662564699

    Chicago Manual of Style (17th edition)

wright1527185662564699
515
© 2018, some rights reserved.Creative Commons License Coupling of Mechanical and Electromagnetic Fields Stimulation for Bone Tissue Engineering by Alyaa I. Aldebs is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Wright State University and OhioLINK.