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Thesis Kelsey Hood OhioLink 091718.pdf (1.26 MB)

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Analytical-Based Methodologies for Monitoring the Uptake, Distribution and Molecular Interaction of Silver Nanoparticles with Human Red Blood Cells

Hood, Kelsey L.
http://rave.ohiolink.edu/etdc/view?acc_num=wright1536181853368728

Abstract Details

2018, Master of Science (MS), Wright State University, Chemistry.
The production and incorporation of silver nanoparticles into consumer products and biomedical technologies has skyrocketed in recent years. Thus, it is vital that the uptake, distribution and molecular interaction of silver nanoparticles (AgNPs) with red blood cells is well studied. In this experiment, citrate capped AgNPs 5-10 nm in diameter were incubated with washed red blood cells in a 5% glucose solution for one hour. Unbound AgNPs were removed via glucose washes prior to quantification with graphite furnace atomic absorption spectroscopy and analysis via Cytoviva hyperspectral microscopy and Raman spectroscopy. It was determined that a high percentage of 48±5% of AgNPs were taken up by the cells. Approximately 70% of the AgNPs taken up were adhered to the cell membrane and the rest were found within the cells. Obvious membrane damage was observed in many of these cells. Hyperspectral data showed interaction of extracellular and intracellular AgNPs with cholesterol, phospholipids or other cell membrane components, and hemoglobin respectively. The intracellular interaction with hemoglobin was confirmed by the enhancement of characteristic hemoglobin Raman shifts. Diminished oxyhemoglobin peaks and enhanced deoxyhemoglobin peaks revealed that the AgNP exposed cells showed a decrease in oxygen binding which could have been a result of intracellular and/or extracellular AgNP interactions. After 24 hours of storage at 4°C, SERS enhancement of >400% proved extracellular AgNPs aggregated forming large clusters of cells. In addition to the proven toxicity of silver ions, the adverse effects seen in the results of this study show that exposure of AgNPs to the circulatory system could pose serious health concerns.
Ioana Pavel , Ph.D. (Advisor)
David Dolson, Ph.D. (Committee Member)
Norma Adragna, Ph.D. (Committee Member)
67 p.
Chemistry
Silver Nanoparticles; SERS; Red blood cells; uptake

Recommended Citations

Citations

  • Hood, K. L. (2018). Analytical-Based Methodologies for Monitoring the Uptake, Distribution and Molecular Interaction of Silver Nanoparticles with Human Red Blood Cells [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1536181853368728

    APA Style (7th edition)

  • Hood, Kelsey. Analytical-Based Methodologies for Monitoring the Uptake, Distribution and Molecular Interaction of Silver Nanoparticles with Human Red Blood Cells. 2018. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1536181853368728.

    MLA Style (8th edition)

  • Hood, Kelsey. "Analytical-Based Methodologies for Monitoring the Uptake, Distribution and Molecular Interaction of Silver Nanoparticles with Human Red Blood Cells." Master's thesis, Wright State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=wright1536181853368728

    Chicago Manual of Style (17th edition)

wright1536181853368728
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© 2018, some rights reserved.Creative Commons License Analytical-Based Methodologies for Monitoring the Uptake, Distribution and Molecular Interaction of Silver Nanoparticles with Human Red Blood Cells by Kelsey L. Hood is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Wright State University and OhioLINK.