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Effect of Endothelial Progenitor Cell-derived Exosomes on High Glucose and Hypoxia/ Reoxygenation-induced Injury of Astrocytes

Halurkar, Manasi Suchit
http://orcid.org/0000-0003-0936-6925
http://rave.ohiolink.edu/etdc/view?acc_num=wright1565806287371214

Abstract Details

2019, Master of Science (MS), Wright State University, Pharmacology and Toxicology.
In this study, we tested the protective effects of EPC-EXs (endothelial progenitor cell derived exosomes) and miR-126 EPC-EXs (microRNA-126 EPC-EXs) on the astrocytes injured by HG (high glucose) plus H/R (hypoxia/reoxygenation) model. At first, we determined the concentration and time dependent uptake of EPC-EXs by astrocytes. It was also found that the EPC-EXs were uptaken via macropinocytosis, caveolin-dependent and clathrin-mediated pathways in astrocytes. Furthermore, the astrocyte cell line was injured through the HG + H/R model. EXs, isolated by ultracentrifugation from the EPC culture supernatant were co-incubated with the injured cells. It was found that EPC-EXs and miR- 126 EPC-EXs decrease apoptosis, lipid peroxidation, oxidative stress and cytotoxicity in the injured cells, hence protecting them.
Ji C. Bihl, M.D., Ph.D. (Advisor)
Adrian M. Corbett, Ph.D. (Committee Member)
Yanfang Chen, M.D., Ph.D. (Committee Member)
80 p.
Pharmacology
Exosome; miR-126; Astrocyte; protective effect; hypoxia; reoxygenation; endothelial progenitor cell

Recommended Citations

Citations

  • Halurkar, M. S. (2019). Effect of Endothelial Progenitor Cell-derived Exosomes on High Glucose and Hypoxia/ Reoxygenation-induced Injury of Astrocytes [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1565806287371214

    APA Style (7th edition)

  • Halurkar, Manasi. Effect of Endothelial Progenitor Cell-derived Exosomes on High Glucose and Hypoxia/ Reoxygenation-induced Injury of Astrocytes. 2019. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1565806287371214.

    MLA Style (8th edition)

  • Halurkar, Manasi. "Effect of Endothelial Progenitor Cell-derived Exosomes on High Glucose and Hypoxia/ Reoxygenation-induced Injury of Astrocytes." Master's thesis, Wright State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=wright1565806287371214

    Chicago Manual of Style (17th edition)

wright1565806287371214
312
© 2019, some rights reserved.Creative Commons License Effect of Endothelial Progenitor Cell-derived Exosomes on High Glucose and Hypoxia/ Reoxygenation-induced Injury of Astrocytes by Manasi Suchit Halurkar is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Wright State University and OhioLINK.