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PT THESIS 2019.pdf (16.18 MB)

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Kinetics of Microvesicle Particle Release in Keratinocytes

Thapa, Pariksha
http://orcid.org/0000-0002-7368-2589
http://rave.ohiolink.edu/etdc/view?acc_num=wright1566504910360327

Abstract Details

2019, Master of Science (MS), Wright State University, Pharmacology and Toxicology.
Microvesicle particles (MVPs) are subcellular particles that could be involved in inter-cellular communication because they carry various bioactive substances including cytokines. Previous studies from our lab has shown that that the lipid mediator Platelet-activating Factor (1-alkyl-2-acetyl-glycerophosphocholine; PAF) and ultraviolet B radiation (UVB) enhances the release of MVP in various cell types like primary keratinocytes, epithelial cell lines, and murine skin. We hypothesized that there may be synergistic increases in MVP release after combination of treatment of keratinocytes with UVB and PAF agonist (CPAF)/phorbol ester (PMA). The combination treatment significantly increases MVP and cytokine release at 4 to 8 hours time points. Imipramine, an acid sphingomyelinase (aSMase) inhibitor blocks MVP and cytokine release which indicates that MVP could be involved in cytokine release. The increased level of TNF-alpha in the supernatant was not present in the MVP but surprisingly there was increased level of anti-inflammatory cytokine, Interleukin 1- receptor antagonist (IL-1ra) inside MVP after UVB treatment than in combination treatment, which could portray potential imbalances between pro-inflammatory cytokines and down regulating responses in exaggerated condition. These studies suggest that MVP could play a role in how keratinocytes respond to UVB.
Jeffrey B. Travers, M.D., Ph.D. (Advisor)
Michael G. Kemp, Ph.D. (Committee Member)
Ji Chen Bihl, M.D., Ph.D. (Committee Member)
137 p.
Pharmacology
Microvesicle Particles; cytokines; Platelet Activating Factor; ultraviolet B radiation; keratinocytes; acid sphingomyelinase; TNF-alpha; Interleukin 1-receptor antagonist

Recommended Citations

Citations

  • Thapa, P. (2019). Kinetics of Microvesicle Particle Release in Keratinocytes [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1566504910360327

    APA Style (7th edition)

  • Thapa, Pariksha. Kinetics of Microvesicle Particle Release in Keratinocytes. 2019. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1566504910360327.

    MLA Style (8th edition)

  • Thapa, Pariksha. "Kinetics of Microvesicle Particle Release in Keratinocytes." Master's thesis, Wright State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=wright1566504910360327

    Chicago Manual of Style (17th edition)

wright1566504910360327
191
© 2019, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.