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AWilcox_FinalThesis_OhioLink.pdf (14.7 MB)

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Silver Nanoparticles: An effective antibacterial agent against Gram-negative bacteria

Wilcox, Ashley M.
http://rave.ohiolink.edu/etdc/view?acc_num=wright1576621225597745

Abstract Details

2019, Master of Science (MS), Wright State University, Chemistry.
A fourth of the world’s population lack access to safe water, thus the need for a more effective water treatment is imperative. Interest in silver nanoparticles (AgNPs) has grown in the last decade. Unlike chlorine, AgNPs do not form disinfection by products (DBPs), making them a prime candidate for drinking water treatment. The main aim of this study was to compare the antibacterial activity of electrochemical silver nanoparticles (eAgNPs-f) of ~5 nm in diameter against well-established pathogens: Escherichia coli (E. coli), Klebsiella variicola (K. variicola), and Pseudomonas aeruginosa (P. aeruginosa) to chlorine and Ag+ for drinking water. This was achieved by determining the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of eAgNPs, which were synthesized electrochemically and then concentrated (eAgNPs-f). The MIC values for E. coli, K. variicola, and P. aeruginosa were 4 ± 3, 3 ± 2, and 3 ± 2 mg/L, respectively. The MBC values for the same bacteria were 4 ± 3, 5 ± 2, and 5 ± 4 mg/L, respectively. When tested against chlorine, the MIC and MBC values increased over 1000-fold. CytoViva Hyperspectral Microscopy demonstrated the eAgNPs-f’s affinity for the cellular membrane of E. coli after 30 minutes and physical cellular damage after 1 hour. Membrane disruption was confirmed through monitoring K+ leakage on ICP-OES. It was found that eAgNPs-f have a rapid and time consistent effect on K+ leakage, when compared to untreated control cells and Ag+. These results suggest that eAgNPs-f containing Ag+ ions are a more effective antibacterial agent than Ag+ alone, or chlorine.
Ioana E. Pavel, Ph.D. (Advisor)
David A. Dolson, Ph.D. (Committee Member)
Steven R. Higgins, Ph.D. (Committee Member)
Marjorie M. Markopoulos, Ph.D. (Committee Member)
52 p.
Chemistry; Nanotechnology
silver nanoparticle; drinking water treatment; electrochemical silver nanoparticles; Escherichia coli; Klebsiella variicola; Pseudomonas aeruginosa; Minimum Inhibitory Concentration; Minimum Bactericidal Concentration; antibacterial agent;

Recommended Citations

Citations

  • Wilcox, A. M. (2019). Silver Nanoparticles: An effective antibacterial agent against Gram-negative bacteria [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1576621225597745

    APA Style (7th edition)

  • Wilcox, Ashley. Silver Nanoparticles: An effective antibacterial agent against Gram-negative bacteria . 2019. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1576621225597745.

    MLA Style (8th edition)

  • Wilcox, Ashley. "Silver Nanoparticles: An effective antibacterial agent against Gram-negative bacteria ." Master's thesis, Wright State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=wright1576621225597745

    Chicago Manual of Style (17th edition)

wright1576621225597745
203
© 2019, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.