Skip to Main Content
 

Global Search Box

 
 
 

ETD Abstract Container

Abstract Header

Genetic Study of Checkpoint Defects of the Mus81-1 Mutant in the Fission Yeast Schizosaccharomyces Pombe.

Abrefa, Darlington Osei
http://rave.ohiolink.edu/etdc/view?acc_num=wright1578464554813491

Abstract Details

2019, Master of Science (MS), Wright State University, Microbiology and Immunology.
In response to various perturbations of DNA replication, the DNA replication checkpoint is activated in eukaryotes to stimulate a cascade of cellular responses that are crucial for maintaining genome stability and cell survival. Defects in the checkpoint pathway result in mutations and genome instability, which is a hallmark for cancers. This study used a genetic approach to identify a mutation in the MMS (methyl methanesulfonate) and UV-sensitive protein Mus81, a DNA repair enzyme that resolves aberrant DNA structures through the homologous recombination pathway. We show that a single missense mutation, identified in fission yeast mus81-1, causes moderate reduction in the phosphorylation levels of the major DNA replication checkpoint proteins Mrc1(Claspin) and Cds1(Chk2) in fission yeast. We also show that the mutation directly affects the DNA repair and the DNA damage checkpoint mediated by Chk1 that causes dramatic cell lethality in mus81-1 mutant upon treatment with the DNA damaging agents: MMS, UV and Bleomycin.
Yong-jie Xu, M.D., Ph.D. (Advisor)
Michael G. Kemp, Ph.D. (Committee Member)
Dawn P. Wooley, Ph.D. (Committee Member)
Nancy J. Bigley, Ph.D. (Committee Member)
50 p.
Genetics; Microbiology; Molecular Biology
DNA replication checkpoint; DNA damage checkpoint; Mus81; DNA repair; genetic mutation; cell cycle; phosphorylation; Mrc1; Cds1; Chk1; Fission yeast mutant; MMS; UV; HU; BLM

Recommended Citations

Citations

  • Abrefa, D. O. (2019). Genetic Study of Checkpoint Defects of the Mus81-1 Mutant in the Fission Yeast Schizosaccharomyces Pombe. [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1578464554813491

    APA Style (7th edition)

  • Abrefa, Darlington. Genetic Study of Checkpoint Defects of the Mus81-1 Mutant in the Fission Yeast Schizosaccharomyces Pombe. 2019. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1578464554813491.

    MLA Style (8th edition)

  • Abrefa, Darlington. "Genetic Study of Checkpoint Defects of the Mus81-1 Mutant in the Fission Yeast Schizosaccharomyces Pombe." Master's thesis, Wright State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=wright1578464554813491

    Chicago Manual of Style (17th edition)

wright1578464554813491
167
© 2019, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.