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Bahir Al-Anbari Thesis.pdf (1.11 MB)

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Characterization of Parvalbumin and Nxph1 Expression in Lumbar Dorsal Root Ganglia by In Situ Hybridization

Al-Anbari, Bahir Rami
http://rave.ohiolink.edu/etdc/view?acc_num=wright1590091377680566

Abstract Details

2020, Master of Science (MS), Wright State University, Anatomy.
Proprioception is a sensory modality that conveys information regarding the body’s movement and position in three-dimensional space. The sensory afferent fibers responsible for this modality, proprioceptors, are a special subtype of mechanoreceptors, the sensory afferent family responsible for movement sensing. It has been shown that proprioceptive neurons undergo several physiological changes after peripheral nerve injury, including perhaps changes in gene expression. In this study, we design riboprobes to use for in situ hybridization to detect two proprioceptive genetic markers, Parvalbumin and Nxph1 in mouse dorsal root ganglia. Tissue samples were prepared from three animal conditions, adult control, sham surgery, and sciatic nerve transection injury. We were successfully able to design the riboprobes needed for specific staining and monitored expression patterns in each condition. Results showed inconclusive staining for the sham surgery and transection injury controls for Nxph1, whereas Parvalbumin showed a reduced, but continued expression after nerve injury.
David R. Ladle, Ph.D. (Advisor)
Patrick M. Sonner, Ph.D. (Committee Member)
Mark M. Rich, M.D., Ph.D. (Committee Member)
59 p.
Nanoscience
Proprioception; proprioceptors; mechanoreceptors; movement sensing; peripheral nerve injury; proprioceptive genetic markers; Parvalbumin; Nxph1

Recommended Citations

Citations

  • Al-Anbari, B. R. (2020). Characterization of Parvalbumin and Nxph1 Expression in Lumbar Dorsal Root Ganglia by In Situ Hybridization [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1590091377680566

    APA Style (7th edition)

  • Al-Anbari, Bahir. Characterization of Parvalbumin and Nxph1 Expression in Lumbar Dorsal Root Ganglia by In Situ Hybridization. 2020. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1590091377680566.

    MLA Style (8th edition)

  • Al-Anbari, Bahir. "Characterization of Parvalbumin and Nxph1 Expression in Lumbar Dorsal Root Ganglia by In Situ Hybridization." Master's thesis, Wright State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=wright1590091377680566

    Chicago Manual of Style (17th edition)

wright1590091377680566
119
© 2020, some rights reserved.Creative Commons License Characterization of Parvalbumin and Nxph1 Expression in Lumbar Dorsal Root Ganglia by In Situ Hybridization by Bahir Rami Al-Anbari is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Wright State University and OhioLINK.