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Delaney Grant Final Thesis Document.pdf (1.39 MB)

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A Novel Method for Analysis of Proprioceptor Sensory Neuron Subtypes in the Mouse Dorsal Root Ganglia

Grant, Delaney C.
http://orcid.org/0000-0001-6178-9410
http://rave.ohiolink.edu/etdc/view?acc_num=wright1620169479989827

Abstract Details

2021, Master of Science (MS), Wright State University, Physiology and Neuroscience.
Proprioceptive sensory neurons encode critical mechanosensory information that helps determine how the body interacts with the outside world and monitors the proper execution of motor movements. Housed in skeletal muscles lie specialized mechanoreceptors that are critical to this feedback loop: muscle spindles supplied by group Ia & group II afferents, and Golgi tendon organs supplied by group Ib afferents relay information regarding changes in muscle force, length, and tension. All three afferent subtypes originate in the muscle and travel to the dorsal root ganglia, relaying information to the central nervous system. GTO and MS proprioceptive afferent subtypes have been identified, traced, and labeled by restrictive RNA and DNA sequencing techniques that eliminate the potential for in vivo and ex vivo analysis. To confirm the identity of different proprioceptive afferent subtypes in the dorsal root ganglia of mice, the present study developed a method of fluorescence tracing using a dextran dye to trace afferents and their origins. By injecting tetramethylrhodamine dextran dye directly into the quadriceps muscle, the muscle spindle proprioceptive afferents selectively transport the dye to the cell body in the DRG demonstrating its origin and classification. Being able to selectively label and trace muscle spindle afferents allows us to accurately collect data on the cells in the DRGs.
David R. Ladle, Ph.D. (Advisor)
Mark M. Rich, M.D., Ph.D. (Committee Member)
Patrick M. Sonner, Ph.D. (Committee Member)
85 p.
Neurobiology; Neurosciences; Physiology
Proprioception; proprioceptors; muscle spindles; Golgi tendon organs; Parvalbumin; movement sensing; proprioceptive sensory neurons; proprioceptive sensory afferents; DRG; dorsal root ganglia; rhodamine;

Recommended Citations

Citations

  • Grant, D. C. (2021). A Novel Method for Analysis of Proprioceptor Sensory Neuron Subtypes in the Mouse Dorsal Root Ganglia [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1620169479989827

    APA Style (7th edition)

  • Grant, Delaney. A Novel Method for Analysis of Proprioceptor Sensory Neuron Subtypes in the Mouse Dorsal Root Ganglia. 2021. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1620169479989827.

    MLA Style (8th edition)

  • Grant, Delaney. "A Novel Method for Analysis of Proprioceptor Sensory Neuron Subtypes in the Mouse Dorsal Root Ganglia." Master's thesis, Wright State University, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=wright1620169479989827

    Chicago Manual of Style (17th edition)

wright1620169479989827
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© 2021, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.