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Identification of Metal Resistance Genes in a Strain of Enterobacter cloacae

Konda, Venkataramana
http://rave.ohiolink.edu/etdc/view?acc_num=ysu1219421291

Abstract Details

2008, Master of Science in Chemistry, Youngstown State University, Department of Chemistry.
A multi-metal resistant strain of Enterobacter cloacae (E. cloacae) grows when exposed to toxic salts of mercury, cadmium, zinc, copper and selenite. In general, bacteria respond to toxic metal concentrations using efflux mechanisms, metal transformation (reduction and oxidation), and sequestration. Transposon mutagenesis was used to generate five selenite sensitive, two zinc sensitive and three cadmium sensitive strains of E. cloacae. DNA sequencing of the mutagenized genes suggested that a polyphosphate kinase, a sporulation domain protein, a Lon protease and Type-II Secretion protein may be involved in selenite resistance. In addition, a P-type ATPase may be involved in Zn resistance. The sporulation domain protein, tyrosine recombinase and Lon protease may be expressed in response to selenite-induced oxidative stress, the polyphosphate kinase may be involved in selenite reduction and processing and the type-II Sec protein may be involved in selenite efflux. The P-type ATPase may be involved in mercury/cadmium/zinc efflux. Finally, the sequence of two cloned PCR fragments indicated that the E. cloacae strain contains genes for copper and mercury resistance. By studying metal-resistance mechanisms, it may be possible to develop strategies to clean metal-contaminated waste sites.
Jonathan Caguiat, PhD (Advisor)
Timothy Wagner, PhD (Committee Member)
Michael Serra, PhD (Committee Member)
126 p.
Biology; Cellular Biology; Genetics; Microbiology; Molecular Biology
Metal resistance genes; Transposon mutagenesis; Polymerase chain reaction

Recommended Citations

Citations

  • Konda, V. (2008). Identification of Metal Resistance Genes in a Strain of Enterobacter cloacae [Master's thesis, Youngstown State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1219421291

    APA Style (7th edition)

  • Konda, Venkataramana. Identification of Metal Resistance Genes in a Strain of Enterobacter cloacae. 2008. Youngstown State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ysu1219421291.

    MLA Style (8th edition)

  • Konda, Venkataramana. "Identification of Metal Resistance Genes in a Strain of Enterobacter cloacae." Master's thesis, Youngstown State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1219421291

    Chicago Manual of Style (17th edition)

ysu1219421291
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© 2008, all rights reserved.
This open access ETD is published by Youngstown State University and OhioLINK.