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Bioaugmentation using Pleurotus ostreatus to Remediate Polycyclic Aromatic Hydrocarbons (PAH) Contaminated River Sediment

Bosiljcic, Gregory Roy
http://rave.ohiolink.edu/etdc/view?acc_num=ysu1220405240

Abstract Details

2008, Master of Science in Biological Sciences, Youngstown State University, Department of Biological Sciences and Chemistry.
The purpose of the study was to determine if polyaromatic hydrocarbon degradation in historically contaminated river sediment could be done when treated with the white-rot fungus Pleurotus ostreatus. P. ostreatus, grown on barley, was added to sediment with various amendments and controls, and incubated in triplicate at 25 °C for 42 days. Treatments included sawdust, shredded newspaper, a nitrogen source, and cyclodextrin. The most effective treatment included the addition of white-rot fungi, sawdust, nitrogen, and cyclodextrin. This treatment showed greater than 50% degradation of 9 our of the 11 PAHs with 95% degradation for benzo fluoranthene. Fungal biomass (total mycelia and metabolically active mycelia) increased in all treatments with added fungi. The greatest increase in fungal biomass appeared in the same treatment with the greatest extent of PAH degradation (from 82 ± 10 m g sediment-1 at time 0 to 374 ± 18 m g sediment-1 at 42 days). These data shown that P. ostreatus is capable of colonizing highly contaminated Mahoning River sediment and degrading the PAHs present. With better optimization of amendments, this approach shows potential for remediating historically contaminated river sediment.
Carl Johnston, PhD (Advisor)
Chester Cooper, PhD (Committee Member)
Roland Riesen, PhD (Committee Member)
40 p.
Biology; Environmental Science; Microbiology
Remediation; White rot fungi; PAH

Recommended Citations

Citations

  • Bosiljcic, G. R. (2008). Bioaugmentation using Pleurotus ostreatus to Remediate Polycyclic Aromatic Hydrocarbons (PAH) Contaminated River Sediment [Master's thesis, Youngstown State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1220405240

    APA Style (7th edition)

  • Bosiljcic, Gregory. Bioaugmentation using Pleurotus ostreatus to Remediate Polycyclic Aromatic Hydrocarbons (PAH) Contaminated River Sediment. 2008. Youngstown State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ysu1220405240.

    MLA Style (8th edition)

  • Bosiljcic, Gregory. "Bioaugmentation using Pleurotus ostreatus to Remediate Polycyclic Aromatic Hydrocarbons (PAH) Contaminated River Sediment." Master's thesis, Youngstown State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1220405240

    Chicago Manual of Style (17th edition)

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© 2008, all rights reserved.
This open access ETD is published by Youngstown State University and OhioLINK.