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Proteomic Characterization of Selenite Resistance in a strain of Enterobacter cloacae

Barasa, Nathaniel Wafula
http://rave.ohiolink.edu/etdc/view?acc_num=ysu1221154755

Abstract Details

2008, Master of Science in Biological Sciences, Youngstown State University, Department of Biological Sciences and Chemistry.
A multimetal resistant strain of Enterobacter cloacae (E. cloacae) grew in the presence of 40mM selenite and demonstrated the ability to transform it into a red precipitate. Established growth curve studies in M-9 minimal salts medium showed that the E. cloacae strain grew in the presence of selenite only when the medium was supplemented with L-cysteine. During early log-phase, selenite or water was added. One hour later, cells were harvested for protein analysis by two dimensional gel electrophoresis (2DGE). Protein profiles of cultures grown under the resistant (cysteine) and sensitive (no cysteine) conditions were compared , and differentially expressed polypeptide spots were excised and sent to Ohio state University for identification by mass spectrometry. The identified proteins were classified under three possible functions: 1) oxidative stress response, 2) selenium processing and 3) selenite efflux. None of the identified proteins indicated a possible role for cysteine in selenite resistance.
Jonathan Caguiat, PhD (Advisor)
Chester Cooper, PhD (Committee Member)
Gary Walker, PhD (Committee Member)
77 p.
Biology; Microbiology
proteomic; selenite resistance; Enterobacter cloacae; Mass Spectrometry; sypro; selenocysteine; oxidative stress

Recommended Citations

Citations

  • Barasa, N. W. (2008). Proteomic Characterization of Selenite Resistance in a strain of Enterobacter cloacae [Master's thesis, Youngstown State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1221154755

    APA Style (7th edition)

  • Barasa, Nathaniel. Proteomic Characterization of Selenite Resistance in a strain of Enterobacter cloacae. 2008. Youngstown State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ysu1221154755.

    MLA Style (8th edition)

  • Barasa, Nathaniel. "Proteomic Characterization of Selenite Resistance in a strain of Enterobacter cloacae." Master's thesis, Youngstown State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1221154755

    Chicago Manual of Style (17th edition)

ysu1221154755
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© 2008, all rights reserved.
This open access ETD is published by Youngstown State University and OhioLINK.