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ysu1232132357.pdf (322.54 KB)
ETD Abstract Container
Abstract Header
The Effect of MV-II-065 on The Phagocytosis of Staphylococcus aureus
Author Info
Royal, Maurice Terrell
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=ysu1232132357
Abstract Details
Year and Degree
2008, Master of Science in Biological Sciences, Youngstown State University, Department of Biological Sciences and Chemistry.
Abstract
Staphylococcus aureus
is a major source of many clinical problems and is associated with an increase in the cost of health care. The bacteria's ability to be resistant to antibiotics remains ever constant.
S. aureus'
cell wall is composed of a capsule that hinders the human body's immune system by preventing phagocytosis of the bacteria. The ineffectiveness of antibiotics and the body's immune system creates the need for alternative methods of treatment for
S. aureus
infections. A potential alternative form of treatment involves the use of mimetics, modified capsular carbohydrates. The goal of these studies was to test the ability of a mimetic, MV-II-065, to block the synthesis of
S. aureus
capsule. To ascertain the effectiveness of the mimetics, they were administered in different concentrations and incubated with
S. aureus
overnight. A decrease in capsule production (26% of solvent control) was indicated by decreased binding of monoclonal antibodies to the bacteria during an ELISA assay. Results of phagocytosis assays were variable, however the mimetics had no effect on bacterial growth rates. Colony counts were performed on bacteria treated with mimetics. These studies demonstrated that incubation with the MV-II-065 altered the light absorbance of the bacteria. Bacteria concentrations standardized by absorbance at 550 nm gave falsely low values in bacteria treated with the mimetics, resulting in higher numbers of bacteria in mimetic treated populations compared to controls. In future studies examining the functional effect of MV-II-065, the bacterial concentrations will have to be verified using colony counts prior to performing phagocytosis assays.
Committee
Diana Fagan, PhD (Advisor)
David Ash, PhD (Committee Member)
John Caguiat, PhD (Committee Member)
Pages
74 p.
Subject Headings
Biology
;
Immunology
;
Microbiology
Keywords
phagocytosis of S. aureus
;
MV-II-065
;
opsonization of S. aureus
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RIS
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Citations
Royal, M. T. (2008).
The Effect of MV-II-065 on The Phagocytosis of Staphylococcus aureus
[Master's thesis, Youngstown State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1232132357
APA Style (7th edition)
Royal, Maurice.
The Effect of MV-II-065 on The Phagocytosis of Staphylococcus aureus.
2008. Youngstown State University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=ysu1232132357.
MLA Style (8th edition)
Royal, Maurice. "The Effect of MV-II-065 on The Phagocytosis of Staphylococcus aureus." Master's thesis, Youngstown State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1232132357
Chicago Manual of Style (17th edition)
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Document number:
ysu1232132357
Download Count:
498
Copyright Info
© 2008, all rights reserved.
This open access ETD is published by Youngstown State University and OhioLINK.