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ysu1254341459.pdf (2.57 MB)
ETD Abstract Container
Abstract Header
Effect of Pleurotus ostreatus on Bioremediation of PAH Contaminated River Sediment
Author Info
Gacura, Matthew David
ORCID® Identifier
http://orcid.org/0000-0003-3437-0483
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=ysu1254341459
Abstract Details
Year and Degree
2009, Master of Science in Biological Sciences, Youngstown State University, Department of Biological Sciences and Chemistry.
Abstract
The purpose of this study was to optimize bioremediation of Mahoning River sediment historically contaminated with polycyclic aromatic hydrocarbons (PAHs) using white rot fungi. Pleurotus ostreatus grown on grain (10% v/v) was added to contaminated sediment amended with sawdust (80% v/v), with and without fungal specific nutritional nitrogen (to enhance fungal growth), and with cyclodextrin (to increase PAH availability). Sediment mixtures were incubated in the dark at 25°C for 6 weeks. Sawdust made the sediment more porous, allowed better colonization by fungi, and did not greatly increase volume. Fungal biomass, determined using fluorescent microscopy, indicated initial fungal colonization but then fungal growth was inhibited, likely by toxic metals or high moisture content in the sediment. Growth of unidentified fungi was observed, especially in treatments amended with nitrogen. Total PAH concentrations (in the order of 100 ppm), analyzed using a gas chromatograph mass spectrometer (GCMS), and significantly decreased ~ 50-60% in all treatments, including sediment only controls within the first two weeks. Thus, aerobic degradation by native bacteria and volatilization were likely responsible for most of the observed decreases in PAH concentrations. High heterogeneity of PAHs in this historically contaminated sediment led to high variance between replicates. There was a slight decrease in 5 ring PAHs associated with sediment inoculated with P. ostreatus and also a slight decrease in total PAH concentrations associated with sediment amended with sawdust and cyclodextrin (with or without P. ostreatus). Increased nitrogen did not enhance PAH degradation. Sediment inoculated with P. ostreatus after two weeks, rather than initially, showed better fungal growth and colonization, but PAH data was not yet available. These data indicate there is great potential for bioremediation of PAH contaminated sediment conditions by stimulating indigenous bacteria under aerobic conditions followed by the addition of white rot fungi. However, further testing and optimization is still required.
Committee
Carl Johnston, PhD (Advisor)
Chester Cooper, PhD (Committee Member)
Jonathan Caguiat, PhD (Committee Member)
Pages
49 p.
Subject Headings
Microbiology
Keywords
Bioremediation
;
PAHs
;
White rot fungi
;
Pleurotus ostreatus
;
Aerobic degradation
;
Polycyclic aromatic hydrocarbons
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Refworks
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Citations
Gacura, M. D. (2009).
Effect of Pleurotus ostreatus on Bioremediation of PAH Contaminated River Sediment
[Master's thesis, Youngstown State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1254341459
APA Style (7th edition)
Gacura, Matthew.
Effect of Pleurotus ostreatus on Bioremediation of PAH Contaminated River Sediment.
2009. Youngstown State University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=ysu1254341459.
MLA Style (8th edition)
Gacura, Matthew. "Effect of Pleurotus ostreatus on Bioremediation of PAH Contaminated River Sediment." Master's thesis, Youngstown State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1254341459
Chicago Manual of Style (17th edition)
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Document number:
ysu1254341459
Download Count:
2,698
Copyright Info
© 2009, all rights reserved.
This open access ETD is published by Youngstown State University and OhioLINK.