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Structural Characteristics of Vpr Protein

Majeti, Jailakshmi Manasa
http://rave.ohiolink.edu/etdc/view?acc_num=ysu1299529352

Abstract Details

2010, Master of Science in Chemistry, Youngstown State University, Department of Chemistry.
Human immunodeficiency virus type 1 (HIV-1) is a retrovirus that is well known to be the causative agent for acquired immunodeficiency syndrome (AIDS). HIV-1 contains many auxiliary genes such as Gag, Pol and Env, regulatory genes such as Tat and Rev and regulatory genes such as Vpr, Vif, Vpx, Vpu and Nef that help regulate its function. Viral protein R (Vpr) is an accessory protein that is expressed from Vpr gene involved in virus replication and plays a key role in the function of HIV-1. There are 96 amino acid residues in the mature form of Vpr which are conserved in HIV-1, HIV-2 and simian immunodeficiency virus (SIV). Vpr is involved in several biological functions like influencing the fidelity of Reverse Transcription process, nuclear import of viral pre integration complex, arrest of G2 phase of the cell cycle of infected human and Simian T cells, inducing apoptosis in human cells and enhancing HIV-1 replication by activation of viral Long Terminal Repeat. Molecular mechanisms involved in the interaction between Vpr and retroviral or cellular proteins are unknown, a structural study of the protein is required to shed light on its numerous biological activities. Our study involved purification of Vpr, verifying the secondary structure of the protein and trying to analyze the structural characteristics of the protein by executing different NMR programs like COSY, NOESY, TOCSY, HSQC. Facilitating the structural investigation of Vpr and gaining a better understanding of structure-function relationship of this protein is the main criteria behind this research.
Ganesaratnam Balendiran, PhD (Advisor)
Sherri Lovelace-Cameron, PhD (Committee Member)
Larry S Curtin, PhD (Committee Member)
59 p.
Chemistry
Vpr protein; NMR; HIV; HPLC

Recommended Citations

Citations

  • Majeti, J. M. (2010). Structural Characteristics of Vpr Protein [Master's thesis, Youngstown State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1299529352

    APA Style (7th edition)

  • Majeti, Jailakshmi Manasa. Structural Characteristics of Vpr Protein. 2010. Youngstown State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ysu1299529352.

    MLA Style (8th edition)

  • Majeti, Jailakshmi Manasa. "Structural Characteristics of Vpr Protein." Master's thesis, Youngstown State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1299529352

    Chicago Manual of Style (17th edition)

ysu1299529352
412
© 2011, all rights reserved.
This open access ETD is published by Youngstown State University and OhioLINK.