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Two-dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) Characterization of Decorin

Brown, Andrew S.
http://rave.ohiolink.edu/etdc/view?acc_num=ysu1311873768

Abstract Details

2011, Master of Science in Biological Sciences, Youngstown State University, Department of Biological Sciences and Chemistry.
Left-ventricular cardiac hypertrophy is a disorder resulting from uncontrolled chronic hypertension. Extensive remodeling of the cardiac extracellular matrix (ECM) through differential expression of ECM proteins allows for the hypertrophied state of the heart to exist and eventually lead to cardiomyopathy. Increases in the levels of collagen in hypertrophied animal samples were noted in a previous study, it is unclear whether is this a result of decreased collagen degradation or increased collagen synthesis. This study aimed to determine the effect of Small Leucine Rich Proteoglycans (SLRPs) which are believed to have a dramatic effect on the degradation of collagen (decreased degradation). The SLRP decorin was characterized via two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) using recombinant decorin. These results showed that decorin is not a peptide that is well suited for 2D-PAGE analysis, and show that another method of analysis must be used in order to characterize and identify in-vivo decorin in animal samples.
Johanna Krontiris-Litowitz, PhD (Advisor)
Diana Fagan, PhD (Committee Member)
Heather Lorimer, PhD (Committee Member)
51 p.
Biology; Biomedical Research; Cellular Biology
decorin; left-ventricular hypertrophy; collagen; small leucine rich proteoglycans

Recommended Citations

Citations

  • Brown, A. S. (2011). Two-dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) Characterization of Decorin [Master's thesis, Youngstown State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1311873768

    APA Style (7th edition)

  • Brown, Andrew. Two-dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) Characterization of Decorin. 2011. Youngstown State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ysu1311873768.

    MLA Style (8th edition)

  • Brown, Andrew. "Two-dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) Characterization of Decorin." Master's thesis, Youngstown State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1311873768

    Chicago Manual of Style (17th edition)

ysu1311873768
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© 2011, all rights reserved.
This open access ETD is published by Youngstown State University and OhioLINK.