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IDENTIFICATION OF A PUTATIVE P-TYPE ATPase INVOLVED IN ZINC AND CADMIUM RESISTANCE IN Enterobacter sp. YSU

Ngendahimana, Valentine M.
http://rave.ohiolink.edu/etdc/view?acc_num=ysu1348844348

Abstract Details

2012, Master of Science in Biological Sciences, Youngstown State University, Department of Biological Sciences and Chemistry.
The Y-12 plant in Oak Ridge Tennessee was involved in manufacturing and storing nuclear weapon for the last 60 years of the 20th century. These activities led to the contamination of the nearby East Fork Poplar creek from which Enterobacter sp. YSU was isolated and screened for metal resistance genes. Three main previously identified metal resistance mechanisms are efflux, detoxification and sequestration. This bacterium was found to be resistant to several heavy metals including zinc, cadmium, mercury, copper, gold, chromium, silver and selenite. The goal of this study was to identify the gene encoding zinc and cadmium resistance in Enterobacter sp. YSU. Two mutants, EI24 and F24 were created using a transposon which disrupted a P-type ATPase believed to be responsible for these resistances. Minimal Inhibitory Concentrations (MICs) of the mutants were carried out to establish the phenotype of the mutants. These MICs revealed that the mutants were sensitive to toxic concentrations of zinc and cadmium while the wildtype bacterium was not. Gene rescue was carried out to rescue the disrupted gene and this was sequenced to determine its identity. BLAST identified the gene as a P-type ATPase with 97% similarity to a zinc/cadmium/mercury/lead translocating P-type ATPase in Enterobacter cloacae.
Jonathan Caguiat, Ph.D. (Advisor)
David Asch, Ph.D. (Committee Member)
Xiangjia Min, Ph.D. (Committee Member)
75 p.
P-type ATPase; Metal resistance; Enterobactoer sp. YSU

Recommended Citations

Citations

  • Ngendahimana, V. M. (2012). IDENTIFICATION OF A PUTATIVE P-TYPE ATPase INVOLVED IN ZINC AND CADMIUM RESISTANCE IN Enterobacter sp. YSU [Master's thesis, Youngstown State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1348844348

    APA Style (7th edition)

  • Ngendahimana, Valentine. IDENTIFICATION OF A PUTATIVE P-TYPE ATPase INVOLVED IN ZINC AND CADMIUM RESISTANCE IN Enterobacter sp. YSU. 2012. Youngstown State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ysu1348844348.

    MLA Style (8th edition)

  • Ngendahimana, Valentine. "IDENTIFICATION OF A PUTATIVE P-TYPE ATPase INVOLVED IN ZINC AND CADMIUM RESISTANCE IN Enterobacter sp. YSU." Master's thesis, Youngstown State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1348844348

    Chicago Manual of Style (17th edition)

ysu1348844348
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© 2012, all rights reserved.
This open access ETD is published by Youngstown State University and OhioLINK.