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Raby Thesis with signature.pdf (862.35 KB)
ETD Abstract Container
Abstract Header
Cloning and Overexpression of Yeast Cystathionine γ-Lyase
Author Info
Raby, Roger Lee, Jr.
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=ysu1355527551
Abstract Details
Year and Degree
2012, Master of Science in Chemistry, Youngstown State University, Department of Chemistry.
Abstract
Cystathionine γ-lyase (CGL) is a protein involved in the conversion of methionine to cysteine as part of the trans-sulfuration pathway. Inactivation of the enzyme, possibly by oxidation of reactive site residues, has been linked to cataractogenesis. This research isolated the cystathionine γ-lyase gene (
CYS3
), modified it with an XhoI and BlpI restriction site and enterokinase sequence (creating a modified
CYS3
,
mCYS3
, gene), and ultimately spliced it into a pET-15b plasmid to create a recombinant plasmid. Then transformed the recombinant plasmid into BL21 E. coli cells. This enabled the production of bacterial cells capable of generating µg amounts of CGL for future metal catalyzed oxidation (MCO) research.
Committee
Michael Serra, PhD (Advisor)
Darryl Mincey, PhD (Committee Member)
Ganesaratnam Balendiran, PhD (Committee Member)
Pages
56 p.
Subject Headings
Biochemistry
;
Chemistry
Keywords
cloning
;
overexpression
;
cataractogenesis
;
cystahionine gamma-lyase
;
enterokinase
;
plasmid
;
cystathionine
;
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Refworks
EndNote
RIS
Mendeley
Citations
Raby, Jr., R. L. (2012).
Cloning and Overexpression of Yeast Cystathionine γ-Lyase
[Master's thesis, Youngstown State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1355527551
APA Style (7th edition)
Raby, Jr., Roger.
Cloning and Overexpression of Yeast Cystathionine γ-Lyase.
2012. Youngstown State University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=ysu1355527551.
MLA Style (8th edition)
Raby, Jr., Roger. "Cloning and Overexpression of Yeast Cystathionine γ-Lyase." Master's thesis, Youngstown State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1355527551
Chicago Manual of Style (17th edition)
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Document number:
ysu1355527551
Download Count:
394
Copyright Info
© 2012, all rights reserved.
This open access ETD is published by Youngstown State University and OhioLINK.