Skip to Main Content
Frequently Asked Questions
Submit an ETD
Global Search Box
Need Help?
Keyword Search
Participating Institutions
Advanced Search
School Logo
Files
File List
Cargal with signature.pdf (1.34 MB)
ETD Abstract Container
Abstract Header
Proteomics and Genomics of Biobutanol Production from
Clostridium beijerinckii
Author Info
Cargal, Timothy Eric
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=ysu1443559123
Abstract Details
Year and Degree
2015, Master of Science in Biological Sciences, Youngstown State University, Department of Biological Sciences and Chemistry.
Abstract
The world consumption of energy is increasing at an exponential rate. Furthermore, the availability of our fuel of choice, fossil fuels, is declining, with estimates that reserves will be gone within fifty years. As such, the search for alternative fuel sources has begun. One promising source of energy is biomass, solar energy stored in the form of sugars by plants. Currently, the focus is on ethanol as a biomass energy source, but it is not without its problems. An emerging alternative to ethanol is butanol, which is chemically more similar to gasoline. Exploiting the Acetone- Butanol-Ethanol Fermentation pathway found in certain species of bacteria can produce butanol. However, this has its own problems in that the butanol is itself toxic to the bacteria. We have identified four potential targets for gene manipulation in order to improve the bacterial resistance to butanol in order to facilitate the production of more butanol. These four genes were G3P, CWBR, PF, and
cspA
. These targets have been analyzed at both the protein expression level and gene expression level. Of these four genes we tested, G3P showed expression levels as expected, PF did not, and the other two were inconclusive due to statistical outliers.
Committee
Gary Walker, PhD (Advisor)
Jonathan Caguiat, PhD (Committee Member)
David Asch, PhD (Committee Member)
Pages
58 p.
Subject Headings
Biology
Keywords
Clostridium beijerinckii
;
Proteomics
;
Genomics
;
Biobutanol
;
Butanol
Recommended Citations
Refworks
EndNote
RIS
Mendeley
Citations
Cargal, T. E. (2015).
Proteomics and Genomics of Biobutanol Production from
Clostridium beijerinckii
[Master's thesis, Youngstown State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1443559123
APA Style (7th edition)
Cargal, Timothy.
Proteomics and Genomics of Biobutanol Production from
Clostridium beijerinckii
.
2015. Youngstown State University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=ysu1443559123.
MLA Style (8th edition)
Cargal, Timothy. "Proteomics and Genomics of Biobutanol Production from
Clostridium beijerinckii
." Master's thesis, Youngstown State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1443559123
Chicago Manual of Style (17th edition)
Abstract Footer
Document number:
ysu1443559123
Download Count:
597
Copyright Info
© 2015, all rights reserved.
This open access ETD is published by Youngstown State University and OhioLINK.