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Identification of a putative ampG ampicillin resistance gene in Stenotrophomonas maltophilia OR02

Ricchiuti, Michelle
http://rave.ohiolink.edu/etdc/view?acc_num=ysu1484670421517116

Abstract Details

2016, Master of Science in Biological Sciences, Youngstown State University, Department of Biological Sciences and Chemistry.
Stenotrophomonas maltophilia OR02 (S. maltophilia 02) is a multidrug resistant bacterium that was isolated from East Fork Poplar Creek in Oak Ridge, Tennessee. S. maltophilia is a nosocomial opportunistic pathogen and is often seen in severely debilitated or immunosuppressed individuals and in the final stages of cystic fibrosis. It tolerates high levels of several metals including cadmium, chromium, copper, gold, lead, mercury, selenium, tellurium and uranium. It is also resistant to many antimicrobial agents such as carbapenems, aminoglycosides, cephalosporins, quinolones, penicillins and many ß-lactam/ß-lactamase inhibitor combinations. Transposon mutagenesis was used to identify an ampicillin sensitive mutant. Minimum inhibitory concentration testing was used to show that the mutant S. maltophilia AJ22 was sensitive to ampicillin. Gene rescue and DNA sequencing were used to obtain the DNA sequence and then the Basic Local Alignment Search Tool was used to determine that it was ampG. ampG is an inner membrane permease involved in transporting murein sacculus degradation products from the periplasm and into the cytosol.
Jonathan Caguiat, PhD (Advisor)
David Asch, PhD (Committee Member)
Xiangjia Min, PhD (Committee Member)
71 p.
Biology; Microbiology
Stenotrophomonas maltophilia; ampG; antibiotic resistance; beta-lactamase

Recommended Citations

Citations

  • Ricchiuti, M. (2016). Identification of a putative ampG ampicillin resistance gene in Stenotrophomonas maltophilia OR02 [Master's thesis, Youngstown State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1484670421517116

    APA Style (7th edition)

  • Ricchiuti, Michelle. Identification of a putative ampG ampicillin resistance gene in Stenotrophomonas maltophilia OR02. 2016. Youngstown State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ysu1484670421517116.

    MLA Style (8th edition)

  • Ricchiuti, Michelle. "Identification of a putative ampG ampicillin resistance gene in Stenotrophomonas maltophilia OR02." Master's thesis, Youngstown State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1484670421517116

    Chicago Manual of Style (17th edition)

ysu1484670421517116
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© 2016, all rights reserved.
This open access ETD is published by Youngstown State University and OhioLINK.