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Using Phage Display to Determine Mesenchymal Stem Cell Contribution to Collagen Synthesis

Kelly, Michael C
http://rave.ohiolink.edu/etdc/view?acc_num=ysu1503615054387659

Abstract Details

2017, Master of Science in Biological Sciences, Youngstown State University, Department of Biological Sciences and Chemistry.
The aim of this project is to ascertain the role of mesenchymal stem cells (MSCs) during wound healing with regard to collagen production. The project utilizes Phage Display to identify specific molecules in MSC supernatant which may augment or impair collagen production during wound healing. MSC supernatant was collected and concentrated via dialysis and lyophilization, then used as the target during Biopanning with the PhD. 7 phage library. Specific phage clones were evaluated for specificity by ELISA, then sequenced to determine amino acid composition. Reverse transcriptase and standard PCR were used to evaluate the quality Collagen I, III, and GAPDH primers for future co-culture experiments. Briefly, MSCs will be co-cultured with fibroblasts in a transwell system that prevents direct contact, but allows paracrine interaction through 4µm pores and shared media. Wounding is then simulated by scratching the fibroblast monolayer. Phage clones demonstrating high specify for MSC supernatant will be added to the co-culture and monolayer allowed to regenerate. Genetic activity of the Collagen I and III genes in fibroblasts will be measured with RT-PCR to elucidate the impact of the phage clone on Collagen production.
Diana Fagan, Ph.D. (Advisor)
Johanna Litowitz, Ph.D. (Committee Member)
David Asch, Ph.D. (Committee Member)
113 p.
Biology; Biomedical Research; Cellular Biology; Molecular Biology
Mesenchymal Stem Cell, Phage Display, Wound Healing, Collagen

Recommended Citations

Citations

  • Kelly, M. C. (2017). Using Phage Display to Determine Mesenchymal Stem Cell Contribution to Collagen Synthesis [Master's thesis, Youngstown State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1503615054387659

    APA Style (7th edition)

  • Kelly, Michael. Using Phage Display to Determine Mesenchymal Stem Cell Contribution to Collagen Synthesis. 2017. Youngstown State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ysu1503615054387659.

    MLA Style (8th edition)

  • Kelly, Michael. "Using Phage Display to Determine Mesenchymal Stem Cell Contribution to Collagen Synthesis." Master's thesis, Youngstown State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1503615054387659

    Chicago Manual of Style (17th edition)

ysu1503615054387659
195
© 2017, all rights reserved.
This open access ETD is published by Youngstown State University and OhioLINK.