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Identification of an L2 ß-lactamase gene from Stenotrophomonas maltophilia OR02

Doyle, Jamielynn
http://rave.ohiolink.edu/etdc/view?acc_num=ysu1528369969169163

Abstract Details

2018, Master of Science in Biological Sciences, Youngstown State University, Department of Biological Sciences and Chemistry.
Stenotrophomonas maltophilia Oak Ridge strain 02 (S. maltophilia 02) was isolated from a heavy metal contaminated stream, East Fork Poplar Creek in Oak Ridge, Tennessee. This strain has high levels of resistance to both metals and antibiotics. All strains of S. maltophilia are gram-negative bacilli. Some strains are opportunistic pathogens that are resistant to high levels of metals and to antibiotics such as cephalosporins, quinolones, carbapenems, penicillins, and ß-lactam/ß-lactamase inhibitor combinations. S. maltophilia 02 contains two ß-lactamase genes, L1 and L2. In this study, the L2 ß-lactamase and its regulator, LysR, were cloned and sequenced. MICs were performed to test for antibiotic resistance against ampicillin, showing resistance even at 1500 µg/ml. In addition, L2 was also found to confer resistance to the antibiotic carbenicillin, but not to cefoxitin. By studying penicillin resistance mechanisms, it may be possible to develop strategies to combat drug resistance in pathogenic strains of S. maltophilia.
Jonathan Caguiat, PhD (Advisor)
David Asch, PhD (Committee Member)
Xiangjia Min, PhD (Committee Member)
44 p.
Biology; Molecular Biology
Antibiotic resistance; Stenotrophomonas maltophilia; Beta lactamase; Ampicillin resistance

Recommended Citations

Citations

  • Doyle, J. (2018). Identification of an L2 ß-lactamase gene from Stenotrophomonas maltophilia OR02 [Master's thesis, Youngstown State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1528369969169163

    APA Style (7th edition)

  • Doyle, Jamielynn. Identification of an L2 ß-lactamase gene from Stenotrophomonas maltophilia OR02. 2018. Youngstown State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ysu1528369969169163.

    MLA Style (8th edition)

  • Doyle, Jamielynn. "Identification of an L2 ß-lactamase gene from Stenotrophomonas maltophilia OR02." Master's thesis, Youngstown State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1528369969169163

    Chicago Manual of Style (17th edition)

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© 2018, all rights reserved.
This open access ETD is published by Youngstown State University and OhioLINK.