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Expression of Genes in Neurospora crassa Outside of the Quinic Acid Gene Cluster During Quinic Acid Metabolism

Savopoulos, John
http://rave.ohiolink.edu/etdc/view?acc_num=ysu152837164807035

Abstract Details

2018, Master of Science in Biological Sciences, Youngstown State University, Department of Biological Sciences and Chemistry.
The quinic acid (qa) gene cluster in Neurospora crassa (N. crassa) consists of two regulatory genes (qa-1F and qa-1S) as well as five structural genes (qa-2, qa-3, qa-4, qa-y, and qa-x) that are transcribed in the presence of quinic acid with suppressed levels of glucose (Greever R.F., et al. 1988). The corresponding activation and repression of this cluster enables the survival of the organism in less than hospitable environments. A research study hypothesized that some 50 genes were under the control of the qa-1F gene and another qa transcription factor. Furthermore, it was shown at the proteomic level in the work of Kayla Brown, Katie Allen, and Dana Tirabassi that glycogen phosphorylase (NCU07027), peptidyl-prolyl cis-trans isomerase (NCU04140), and NCU08332 Hex-1: Woronin Body Coding Protein all had heightened expression at the proteomic level in the presence of quinic acid. Therefore, the scope of this work aimed to analyze these three genes to determine their levels of transcription in the presence of quinic acid (utilizing qRT-PCR), and to determine if they are related to the quinic acid gene cluster. The results indicate that heightened glycogen phosphorylase expression seen in prior works is not related to quinic acid metabolism and is not a result of starvation. However, it is predicted that the cell may be holding on to this protein for other purposes not related to the scope of this work. Peptidyl prolyl cis-trans isomerase appeared to not be related to the quinic acid gene cluster, but rather showed heightened expression as a result of starvation. Last, the NCU08332 Hex-1 Woronin body major protein gene appeared to have a direct relation to quinic acid metabolism and the quinic acid gene cluster. Furthermore, bioinformatics analyses presented also supported this assumptions.
David Asch, PhD (Advisor)
Jonathan Caguiat, PhD (Committee Member)
Xiangjia Min, PhD (Committee Member)
98 p.
Bioinformatics; Biology; Genetics; Molecular Biology
Neurospora crassa; Quinic acid metabolism; qRT-PCR; Hex-1 woronin body protein gene

Recommended Citations

Citations

  • Savopoulos, J. (2018). Expression of Genes in Neurospora crassa Outside of the Quinic Acid Gene Cluster During Quinic Acid Metabolism [Master's thesis, Youngstown State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ysu152837164807035

    APA Style (7th edition)

  • Savopoulos, John. Expression of Genes in Neurospora crassa Outside of the Quinic Acid Gene Cluster During Quinic Acid Metabolism. 2018. Youngstown State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ysu152837164807035.

    MLA Style (8th edition)

  • Savopoulos, John. "Expression of Genes in Neurospora crassa Outside of the Quinic Acid Gene Cluster During Quinic Acid Metabolism." Master's thesis, Youngstown State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=ysu152837164807035

    Chicago Manual of Style (17th edition)

ysu152837164807035
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This open access ETD is published by Youngstown State University and OhioLINK.