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Designing a Two Component System for Enzyme Immobilization Using a Modified Chitosan Support

Mioro, Miriam Kanyua
http://rave.ohiolink.edu/etdc/view?acc_num=ysu15946615388307

Abstract Details

2020, Master of Science in Chemistry, Youngstown State University, Department of Chemistry.
Immobilized multienzyme systems catalyzing cascade reactions have shown to be an effective industrial strategy. These systems reduce production cost by increasing both reusability and stability of enzymes under extreme conditions. Development of a stable support for enzyme immobilization further improves the process. The objective of this study was to improve lactose hydrolysis by designing a two-component system comprised of lactase and glucose oxidase immobilized independently on a modified chitosan support. The structural stability of chitosan was enhanced by addition of fortifying agents such as activated charcoal, silica, and magnetic particles followed by crosslinking beads with glutaraldehyde. Lactase was immobilized on chitosan-magnetic beads, chitosan-charcoal beads, and chitosan-silica beads. Glucose oxidase was immobilized on chitosan-charcoal beads and chitosan-silica beads. The physical properties, immobilization efficiency, and enzymatic activity were determined for each immobilized enzyme. Both enzymes, lactase and glucose oxidase, retained their activity after immobilization. Assays performed with the combinations of the chitosan beads indicated that the immobilized enzymes are capable of catalyzing two sequential reactions - lactose hydrolysis and glucose oxidation.
Nina Stourman, PhD (Advisor)
Michael Serra , PhD (Committee Member)
Douglas Genna , PhD (Committee Member)
88 p.
Biochemistry; Chemistry
Enzyme Immobilization; Immobilized Multienzyme Systems; Lactose Hydrolysis; Chitosan Support; Lactase; Glucose oxidase

Recommended Citations

Citations

  • Mioro, M. K. (2020). Designing a Two Component System for Enzyme Immobilization Using a Modified Chitosan Support [Master's thesis, Youngstown State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ysu15946615388307

    APA Style (7th edition)

  • Mioro, Miriam. Designing a Two Component System for Enzyme Immobilization Using a Modified Chitosan Support. 2020. Youngstown State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ysu15946615388307.

    MLA Style (8th edition)

  • Mioro, Miriam. "Designing a Two Component System for Enzyme Immobilization Using a Modified Chitosan Support." Master's thesis, Youngstown State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ysu15946615388307

    Chicago Manual of Style (17th edition)

ysu15946615388307
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This open access ETD is published by Youngstown State University and OhioLINK.