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Stiffness Gradient Scaffolds as an In Vitro Model for Stem Cell Based Cardiac Cell Therapy

Patel, Kunal
http://rave.ohiolink.edu/etdc/view?acc_num=akron1386725736

Abstract Details

2013, Master of Science in Engineering, University of Akron, Biomedical Engineering.
Mesenchymal stem cell (MSC) therapy has been extensively studied as a viable approach for the treatment of myocardial infarction over the past decade. Mechanical cues have been implicated as a means for stem cell differentiation, suggesting that the stiffness of the extracellular matrix plays an important role in regulating cell behavior. Post-infarction, the myocardium experiences a time-dependent change in stiffness that ranges from flexible healthy tissue to rigid scar tissue. In order to study the effects of MSC implantation into an ischemic heart, it is necessary to develop a scaffold that can mimic the altered myocardium elasticity of the peri-infarct zone. In this study, (1) we described a practical method to develop a scaffold with tunable mechanical properties and characterize the physical properties of varying stiffness gradient strength scaffolds that mimic the peri-infarct zone at various time points post-infarction, and (2) analyzed subsequent MSC behavior on the scaffolds including morphology, proliferation, and migration. The mechanical environment was modeled through a PEG/Collagen composite scaffold with a gradient of mechanical stiffness. By tuning the range of stiffness to that of healthy myocardium to scar tissue, the scaffold holds promise in providing a better understanding of MSC based cardiac cell therapy.
Ge Zhang, Dr. (Advisor)
Rebecca Willits, Dr. (Committee Member)
Gang Cheng, Dr. (Committee Member)
107 p.
Biomedical Engineering; Biomedical Research

Recommended Citations

Citations

  • Patel, K. (2013). Stiffness Gradient Scaffolds as an In Vitro Model for Stem Cell Based Cardiac Cell Therapy [Master's thesis, University of Akron]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=akron1386725736

    APA Style (7th edition)

  • Patel, Kunal. Stiffness Gradient Scaffolds as an In Vitro Model for Stem Cell Based Cardiac Cell Therapy. 2013. University of Akron, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=akron1386725736.

    MLA Style (8th edition)

  • Patel, Kunal. "Stiffness Gradient Scaffolds as an In Vitro Model for Stem Cell Based Cardiac Cell Therapy." Master's thesis, University of Akron, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=akron1386725736

    Chicago Manual of Style (17th edition)

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© 2013, all rights reserved.
This open access ETD is published by University of Akron and OhioLINK.