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The Effects of Ellagic Acid on Insulin-Like Growth Factor Binding Protein-2 in Human Prostate Cancer Cells

Okeke, Joy C.

Abstract Details

2006, Master of Science (MS), Bowling Green State University, Family and Consumer Sciences/food and Nutrition.
Ellagic acid (EA), a dietary phenolic compound found in berries and nuts, elicits important cellular activities such as apoptosis and cell cycle arrest in a variety of cancer cells. Little is known of EA effects on androgen-dependent prostate cancer cells. The purpose of the present study was to determine the in vitro effects of EA on proliferation of human prostate cancer (LNCaP) cells. Additionally, the effect of EA exposure (0.1-10 uM) on concentrations of insulin-like growth factor binding protein-2 (IGFBP-2), a growth factor implicated in prostate cancer, was determined. Cell proliferation was assessed after 1-3 days of exposure to EA (0.03-100 uM), media alone (control), or 0.016% DMSO (vehicle) by counting cells or estimating mitochondrial activity using a cell viability assay (MTS, Promega, Madison, WI). IGFBP-2 secreted into culture media was determined by an enzyme-linked immunosorbant assay (R&D Systems, Minneapolis, MN). EA significantly inhibited cell proliferation of LNCaP cells in a dose-dependent manner. Cell count and MTS data both demonstrated a significant decrease in cell growth for concentrations of EA greater than 0.1 uM compared to vehicle (p<0.05). Cell counts were 89%, 72%, 68%, 38%, and 29% of control for 0.1, 1, 2, 10, and 100 uM EA exposure, respectively. Little change in cell viability occurred within the first 24 h for cells exposed to 0.03 to 50 uM EA; however, marked reduction in cell viability after 24 h at 100 uM EA was observed. IGFBP-2 concentrations were significantly less for cells exposed to 10 uM EA compared to vehicle (8.7, 5.1 ng/mL/500,000 cells, respectively, p<0.05). IGFBP-2 concentrations were lower for 2 uM and 1 uM EA cells as well (7.3, 8.9 ng/mL/500,000 cells), but not statistically different than controls. The present study demonstrated dose-dependent effects of EA in decreasing cell proliferation and suppressing secretion of IGFBP-2 in prostate cancer cells. Doses of EA used in the current study were lower than previous reports using other cancer cell lines, and approximate to EA concentrations reported in human serum. The potential benefits of modifying cancer cell proliferation and growth factors, such as IGFBP-2 via dietary bioactive compounds such as EA are promising and warrant further investigation.
M. Sue Houston (Advisor)
66 p.

Recommended Citations

Citations

  • Okeke, J. C. (2006). The Effects of Ellagic Acid on Insulin-Like Growth Factor Binding Protein-2 in Human Prostate Cancer Cells [Master's thesis, Bowling Green State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1162343994

    APA Style (7th edition)

  • Okeke, Joy. The Effects of Ellagic Acid on Insulin-Like Growth Factor Binding Protein-2 in Human Prostate Cancer Cells. 2006. Bowling Green State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1162343994.

    MLA Style (8th edition)

  • Okeke, Joy. "The Effects of Ellagic Acid on Insulin-Like Growth Factor Binding Protein-2 in Human Prostate Cancer Cells." Master's thesis, Bowling Green State University, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1162343994

    Chicago Manual of Style (17th edition)