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DEVELOPMENT OF LC-MS/MS ASSAYS FOR URINE DRUG TESTING AND QUANTIFICATION OF HUMAN STOOL HEMOGLOBIN FOR COLORECTAL CANCER SCREENING

Alagandula, Ravali
http://rave.ohiolink.edu/etdc/view?acc_num=csu1513598216213184

Abstract Details

2017, Doctor of Philosophy in Clinical-Bioanalytical Chemistry, Cleveland State University, College of Sciences and Health Professions.
Mass Spectrometry allows the accurate determination and quantitation of drugs and proteins with high sensitivity and specificity, making it a powerful method to provide insights into various diseases using biological samples. My dissertation research focuses on the development of mass spectrometry-based methods to quantitate drugs and protein biomarkers in clinical specimen. The background on the development of robust mass spectrometry-based methods to quantify drugs and proteins in biological samples and various sample preparation techniques are described in Chapter I. My dissertation research is composed of two parts. In the first part, I developed three mass spectrometric methods to quantitate several important drugs related to pain management and drug abuse, solving the low-throughput problem encountered in clinical labs. Chapter II discusses the method developed for quantitating phenobarbital (a pain management drug) in human urine. Chapter III describes the method developed for quantitate ethyl-glucuronide (an alcohol biomarker) in human urine. In Chapter IV, I describe a method for quantification of phenobarbital and ethyl-glucuronide in urine using one MS/MS method. We have demonstrated that these methods developed have a throughput of ~700 samples/per day, much higher than the throughput of current methods. In the second part, my research focused on the development and application of a novel immunocapture-MRM method for quantitation of protein biomarkers in stool. Immunocapture-MRM combines immunoassays with mass spectrometry, taking advantages of targeted enrichment of protein biomarkers from complex clinical specimen using immunoassay and specific quantitation by mass spectrometry. Currently, less quantitative elution and digestion of the proteins captured by immunoassay have been a major problem associated with immunocapture-MRM based methods. The part of my research was devoted to the development of methods to solve this problem. Chapter V describes new methods I developed, which enable the quantitative elution and digestion of proteins captured by immunoassay. In Chapter VI, I discuss the development of an immunocapture-MRM method for quantitation of stool proteins and application of this assay to analyze stool samples.
Baochuan Guo, Ph.D. (Advisor)
Aimin Zhou, Ph.D. (Committee Member)
Xiang Zhou, Ph.D. (Committee Member)
John F Turner, Ph.D. (Committee Member)
Chandrasekhar Kothapalli, Ph.D. (Committee Member)
284 p.
Chemistry

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Citations

  • Alagandula, R. (2017). DEVELOPMENT OF LC-MS/MS ASSAYS FOR URINE DRUG TESTING AND QUANTIFICATION OF HUMAN STOOL HEMOGLOBIN FOR COLORECTAL CANCER SCREENING [Doctoral dissertation, Cleveland State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=csu1513598216213184

    APA Style (7th edition)

  • Alagandula, Ravali. DEVELOPMENT OF LC-MS/MS ASSAYS FOR URINE DRUG TESTING AND QUANTIFICATION OF HUMAN STOOL HEMOGLOBIN FOR COLORECTAL CANCER SCREENING . 2017. Cleveland State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=csu1513598216213184.

    MLA Style (8th edition)

  • Alagandula, Ravali. "DEVELOPMENT OF LC-MS/MS ASSAYS FOR URINE DRUG TESTING AND QUANTIFICATION OF HUMAN STOOL HEMOGLOBIN FOR COLORECTAL CANCER SCREENING ." Doctoral dissertation, Cleveland State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=csu1513598216213184

    Chicago Manual of Style (17th edition)

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This open access ETD is published by Cleveland State University and OhioLINK.
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