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Schilling_MS thesis 1.26.21.pdf (3.68 MB)

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Non-Mendelian Inheritance in C. elegans: A Violation of The Law of Independent Assortment

Schilling, Taylor
http://rave.ohiolink.edu/etdc/view?acc_num=csu1611776149127827

Abstract Details

2020, Master of Science in Biology, Cleveland State University, College of Sciences and Health Professions.
Mendel’s laws of inheritance can predict typical genotypic frequencies in subsequent generations. A modern formation of the second law, the Law of Independent Assortment, states that alleles determining different traits are inherited independently. However, in Caenorhabditis elegans males, extrachromosomal DNA arrays and autosomes containing integrated multicopy DNA arrays segregate away from the single male X chromosome, appearing more frequently in male (XO) than hermaphrodite (XX) offspring. For example, we observed that 80% of progeny inheriting a patroclinous copy of chromosome III balancer qC1[nIs281] are males and 20% are hermaphrodites instead of the expected 1:1 ratio of each sex as predicted by Mendel’s laws. We also performed several crosses with different extrachromosomal and integrated arrays (oxEx229, mIs10, ccIs4251, syIs44) and analyzed the inheritance patterns in the offspring to confirm patterns of male biased inheritance. The largest deviations from expected Mendelian frequencies resulted from crosses in which the array contained X chromosome homology. To investigate the mechanism, we performed a forward genetic screen to identify mutations that disrupt non-Mendelian inheritance of the qC1[nIs281] balancer, shifting its transmission back to the expected ratio of 50% males and 50% hermaphrodites. Identifying genes required for non-Mendelian inheritance will define mechanisms that drive evolutionary change. Finally, we tested combinations of probes for FISH that will help visualize the unusual dependent assortment of chromosome III, containing an integrated array, and the X chromosome in future experiments.
Aaron Severson, PhD (Advisor)
Valentin Boerner, PhD (Committee Member)
Bibo Li, PhD (Committee Member)
Biology; Genetics; Molecular Biology

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Citations

  • Schilling, T. (2020). Non-Mendelian Inheritance in C. elegans: A Violation of The Law of Independent Assortment [Master's thesis, Cleveland State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=csu1611776149127827

    APA Style (7th edition)

  • Schilling, Taylor. Non-Mendelian Inheritance in C. elegans: A Violation of The Law of Independent Assortment . 2020. Cleveland State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=csu1611776149127827.

    MLA Style (8th edition)

  • Schilling, Taylor. "Non-Mendelian Inheritance in C. elegans: A Violation of The Law of Independent Assortment ." Master's thesis, Cleveland State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=csu1611776149127827

    Chicago Manual of Style (17th edition)

csu1611776149127827
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This open access ETD is published by Cleveland State University and OhioLINK.