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INVESTIGATION OF THE BIOPHYSICS OF LIPID DROPLETS

Mirheydari, Mona Sadat
http://rave.ohiolink.edu/etdc/view?acc_num=kent1498862985023767

Abstract Details

2017, PHD, Kent State University, College of Arts and Sciences / Department of Physics.
Lipid droplets (LDs) are cell organelles which exist in almost all eukaryote cells. In addition to storing energy, they also take part in other biological processes like building new membrane, synthesizing steroid hormones, and lipid signaling. Malfunction in any of these processes can lead to diseases like diabetes and other obesity-related syndromes. Recently, there have been many studies looking at the cell biology of LDs. However, there is not yet much known about their biophysics and structure. This work aims at acquiring insight about the interaction of lipid droplet binding proteins with the LDs and in particular the phospholipid monolayer. Several different proteins are associated with the surface of human lipid droplets. My work focusses on perilipin 3, also known as TIP 47, which is one of the common LD associated proteins. The different parts of the protein may play different roles. The amphipathic a-helix bundle is a region in the protein which is likely important for proper function of the protein. Such a-helical bundles are found in other proteins such as apoE and apoLp-III (both apolipoproteins). They form a common motif in neutral lipid particle binding proteins. The exact function of the helix bundle in perilipin 3 is unknown, but given that the entire structure of apoLp-III is such a bundle it is likely to be crucial for the function of the protein. As there is less information available about how these helices function in lipid interaction, we study the behavior of amphipathic a-helix bundle proteins in interaction with phospholipid monolayer. Our study has two parts. One is studying the protein interaction at the air/water interface, which is the simplest system to control. Secondly, we look at these interactions at the oil-lipid- water interface, which is more physiologically relevant and experimentally more challenging.
Edgar E. Kooijman (Advisor)
Elizabeth K. Mann (Committee Chair)
John Portman (Committee Member)
Colleen Novak (Committee Member)
Hamza Balci (Committee Member)
Antal Jakli (Committee Member)
299 p.
Biochemistry; Biophysics; Physics

Recommended Citations

Citations

  • Mirheydari, M. S. (2017). INVESTIGATION OF THE BIOPHYSICS OF LIPID DROPLETS [Doctoral dissertation, Kent State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=kent1498862985023767

    APA Style (7th edition)

  • Mirheydari, Mona Sadat. INVESTIGATION OF THE BIOPHYSICS OF LIPID DROPLETS. 2017. Kent State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=kent1498862985023767.

    MLA Style (8th edition)

  • Mirheydari, Mona Sadat. "INVESTIGATION OF THE BIOPHYSICS OF LIPID DROPLETS." Doctoral dissertation, Kent State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=kent1498862985023767

    Chicago Manual of Style (17th edition)

kent1498862985023767
595
© 2017, all rights reserved.
This open access ETD is published by Kent State University and OhioLINK.