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HADEEL ALLAYEITH 2020.pdf (4.73 MB)

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Zinc-Based Nanoparticles Prepared by a Top-Down Method Exhibit Extraordinary Antibacterial Activity Against Both Pseudomonas aeruginosa and Staphylococcus aureus

Allayeith, Hadeel K
http://rave.ohiolink.edu/etdc/view?acc_num=kent1594685506894496

Abstract Details

2020, PHD, Kent State University, College of Arts and Sciences / Department of Chemistry and Biochemistry.
Zinc is an essential element for life, and its vital benefit for human health is well known in the field of food and nutrition. Due to their low toxicity and high biosafety, zinc compounds have been used very early by ancient people to cure wounds and eye sores. Furthermore, zinc as a promising antimicrobial agent has been confirmed by a series of recent reports. Thus far, most studies of its antimicrobial activity have been focused on ZnO NPs prepared by the conventional bottom-up methods such as solution preparation, hydrothermal synthesis, to name but a few. As the result, the reported minimal inhibitory concentration (MIC) values of these NPs range from μM to mM. The general mechanism of metal-based NPs is widely debated in the literature. Generally, ZnO-based NPs can produce reactive oxygen species (ROS) that lead to the damage of protein, membrane and DNA of bacteria. Another path of antimicrobial activity is that ZnO-based NPs can release metal ions to disturb the electron transport chain and regulate the bacterial metabolic process. The Zn2+ ion is an essential ion for many microorganisms due to its involvement in various cellular reactions, but at higher concentration it becomes cytotoxic. Owing to their size and varied morphological features, nanoparticles can facilitate their interactions with biomolecules. Based on the previous mentioned concepts, we have successfully synthesized and investigated the antibacterial activity of four types of nanoparticles based on different zinc compounds using the top-down method to prepare dispersions of polymer-coated different Zn-based NPs with the aid of ultrasound energy. We have successfully developed the broad-spectrum Zn-based NPs against both the Gram-negative bacteria and Gram-positive bacteria. We have found that this novel top-down method for preparing Zn NPs using sonication can produce ultrafine Zn-based NPs with the reduced MIC values. Such NPs can easily rupture the cellular wall. Particularly notable is the fact that all types of these zinc-based NPs prepared from the current technique have showed no sign of drug development in Gram negative P. aeruginosa after 30 cycles of treatment using the sub-inhibitory concentration. Moreover, both the ciprofloxacin-resistant and silver-resistant bacterial mutants are susceptible to all the NPs reported in this study and do not develop drug resistance after 30 cycles of treatment. That new discovery can be used in the future for controlling and treating different infectious caused by multi drug resistant bacteria issues especially that caused the biofilm problems caused chronic infection. Our studies have also demonstrated that all these Zn-based NPs show promising results regarding the cytotoxicity in two types of mammalian cells, pointing to the potential of developing such NPs as safe drugs for clinical use.
Songping Huang (Advisor)
143 p.
Chemistry
Zn-based Nanoparticles; Antibacterial activities; Cytotoxicity; Top-down method

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Citations

  • Allayeith, H. K. (2020). Zinc-Based Nanoparticles Prepared by a Top-Down Method Exhibit Extraordinary Antibacterial Activity Against Both Pseudomonas aeruginosa and Staphylococcus aureus [Doctoral dissertation, Kent State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=kent1594685506894496

    APA Style (7th edition)

  • Allayeith, Hadeel. Zinc-Based Nanoparticles Prepared by a Top-Down Method Exhibit Extraordinary Antibacterial Activity Against Both Pseudomonas aeruginosa and Staphylococcus aureus. 2020. Kent State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=kent1594685506894496.

    MLA Style (8th edition)

  • Allayeith, Hadeel. "Zinc-Based Nanoparticles Prepared by a Top-Down Method Exhibit Extraordinary Antibacterial Activity Against Both Pseudomonas aeruginosa and Staphylococcus aureus." Doctoral dissertation, Kent State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=kent1594685506894496

    Chicago Manual of Style (17th edition)

kent1594685506894496
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This open access ETD is published by Kent State University and OhioLINK.