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Analytical method development and stability indicating studies of novel anticancer compounds IND-2, BAPT-27 and CAST-1000

Giri, Paras Mani

Abstract Details

2020, Master of Science in Pharmaceutical Science (MSP), University of Toledo, Pharmaceutical Sciences (Industrial Pharmacy).
IND-2 is a pyrimido pyrazolo quinoline-derivative compound that has the potential to treat colon cancer. A simple and reliable high-performance liquid chromatographic (HPLC) method was developed for the analysis of IND-2. Stability indicating studies of IND-2 were conducted via forced degradation by subjecting IND-2 to acidic/basic hydrolysis conditions, oxidation, and thermal stress. The mobile phase consisted of water and acetonitrile (80:20, v/v) both containing 0.1% formic acid. The analytical method was validated according to ICH guidelines. The developed analytical method was specific since all the IND-2 degradant peak purity angle was less than the threshold angle. The method was accurate and reliable since the intra-day and inter-day repeatability value was less than 2% and the recovery value was between 95% and 105%. The intrinsic stability of IND-2 varied among various stress conditions. IND-2 is very unstable in basic condition, whereas unstable under thermal stress. It is quite stable in acidic environment and to oxidation. BAPT-27 is a 4-pyridinyl ring containing compound with in vitro anti-colon cancer. A simple and reliable high-performance liquid chromatographic (HPLC) method was developed for the quantitative analysis of BAPT-27. Stability indicating studies of BAPT-27 was conducted via forced degradation by subjecting to BAPT-27 to acidic/basic hydrolysis, oxidation, and thermal stress. The developed method was validated according to ICH guidelines. The mobile phase consisted of water and acetonitrile (60:40, v/v) both containing 0.1% formic acid. The analytical method was specific since all the BAPT-27 degradant peak purity angle was less than the threshold angle. The method was accurate and reliable since the intra-day and inter-day repeatability value was less than 2% and the recovery value was between 95% and 105%. The intrinsic stability of drug varied among various stress conditions. BAPT-27 is unstable in acidic condition and to oxidation whereas quite stable in basic environment and to thermal stress. CAST 1000 is a 4-pyridinyl ring containing compound that has been shown to possess anti-cancer activity against various cancer cell lines. A simple and reliable high-performance liquid chromatographic (HPLC) method was developed for the analysis of CAST-1000. Stability indicating studies of CAST-1000 was conducted via forced degradation by subjecting drug to acidic/basic environment, hydrolysis condition, oxidative condition, and thermal stress. The mobile phase consisted of water and acetonitrile (40:60, v/v) both containing 0.1% formic acid. The developed method was validated according to ICH guidelines. The analytical method was specific since all the CAST-100 degradant peak purity angle was less than the threshold angle. The method was accurate and reliable since the intra-day and inter-day repeatability value was less than 2% and the recovery value was between 95% and 105%. The intrinsic stability of the drug varied among various stress conditions. CAST 1000 is quite stable in acidic/basic condition and oxidative condition whereas quite stable in thermal condition.
Jerry Nesamony (Committee Chair)
Gabriella Baki (Committee Member)
Caren Steinmiller (Committee Member)
67 p.

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Citations

  • Giri, P. M. (2020). Analytical method development and stability indicating studies of novel anticancer compounds IND-2, BAPT-27 and CAST-1000 [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=mco1588799703498036

    APA Style (7th edition)

  • Giri, Paras. Analytical method development and stability indicating studies of novel anticancer compounds IND-2, BAPT-27 and CAST-1000. 2020. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=mco1588799703498036.

    MLA Style (8th edition)

  • Giri, Paras. "Analytical method development and stability indicating studies of novel anticancer compounds IND-2, BAPT-27 and CAST-1000." Master's thesis, University of Toledo, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=mco1588799703498036

    Chicago Manual of Style (17th edition)