Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


miami1218130798.pdf (326.49 KB)

ETD Abstract Container

Abstract Header

THE EFFECTS OF ORPHANIN FQ/NOCICEPTIN (OFQ/N) DELETION ON THE HYPOTHALAMIC-PITUITARY-ADRENAL (HPA) AXIS ACTIVITY AND PROLACTIN RESPONSE TO STRESS

Zullig, Kelly
http://rave.ohiolink.edu/etdc/view?acc_num=miami1218130798

Abstract Details

2008, Doctor of Philosophy, Miami University, Zoology.
These studies were conducted to examine the role of Orphanin FQ/Nociceptin (OFQ/N) in regulating hypothalamic pituitary adrenal (HPA) axis activity and modulating prolactin (PRL) secretion in response to stress. First, studies were conducted to characterize knockout mice under resting conditions and explore possible compensatory mechanisms that may have occurred in OFQ/N knockout animals. There were no differences between knockout and wild-type animals in circulating corticosterone (CORT) or PRL levels, in prolactin receptors (PRL-R) in the choroid plexus, or hypothalamic levels of nociceptin (NOP) or delta receptors (DOP). Knockout males had significantly less mu receptor (MOR) mRNA but this did not translate into a protein difference. Knockout males and females had significantly less kappa receptor protein (KOP) than wild-type animals. OFQ/N injection still produced a significant increase in PRL secretion in knockout animals, indicating that they have functional NOP. There were no genotype differences in HPA axis activation, as indicated by increased CORT levels, or in the PRL secretory response to restraint, orbital shaker or platform shaker stress. This indicates that OFQ/N is not essential for the neuroendocrine response to stress. Further, in males OFQ/N did not affect the animals' ability to habituate to platform shaker stress, signifying that OFQ/N is also not crucial for adaptation to a homotypic stressor. There were clear differences between CORT and PRL in the magnitude and time course of response to the stressors applied. Stressor dependent differences were also seen within the individual CORT and PRL responses. This was not surprising, given the complexity of the stress response and the numerous pathways involved. The magnitude, time course, and even the experimental environment of stressors must be carefully considered when interpreting results of studies involving stress paradigms.
Phyllis Callahan, PhD (Advisor)
James Janik, PhD (Committee Member)
Emily Murphree, PhD (Committee Member)
Katia Del Rio-Tsonis, PhD (Committee Member)
Paul James, PhD (Committee Member)
93 p.
Biology; Zoology
opioids; opiates; gender; corticosterone; opiate receptors; anesthesia; adaptation; habituation

Recommended Citations

Citations

  • Zullig, K. (2008). THE EFFECTS OF ORPHANIN FQ/NOCICEPTIN (OFQ/N) DELETION ON THE HYPOTHALAMIC-PITUITARY-ADRENAL (HPA) AXIS ACTIVITY AND PROLACTIN RESPONSE TO STRESS [Doctoral dissertation, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1218130798

    APA Style (7th edition)

  • Zullig, Kelly. THE EFFECTS OF ORPHANIN FQ/NOCICEPTIN (OFQ/N) DELETION ON THE HYPOTHALAMIC-PITUITARY-ADRENAL (HPA) AXIS ACTIVITY AND PROLACTIN RESPONSE TO STRESS. 2008. Miami University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1218130798.

    MLA Style (8th edition)

  • Zullig, Kelly. "THE EFFECTS OF ORPHANIN FQ/NOCICEPTIN (OFQ/N) DELETION ON THE HYPOTHALAMIC-PITUITARY-ADRENAL (HPA) AXIS ACTIVITY AND PROLACTIN RESPONSE TO STRESS." Doctoral dissertation, Miami University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=miami1218130798

    Chicago Manual of Style (17th edition)

miami1218130798
335
© 2008, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.